11-Hydroxyandrostenedione Test for Adrenal Gland Function

What is this test?

The 11-Hydroxyandrostenedione test measures the amount of a hormone called 11-Hydroxyandrostenedione in your blood. Your adrenal glands are small glands located on top of each kidney, and they produce this hormone along with other important hormones. To stimulate them, a healthcare professional gives you an injection of corticotropin, also called ACTH. Your blood is then collected exactly 1 hour later to show how your adrenal glands responded to the stimulation.

Why do I need this test?

Your doctor may order this test when they suspect that your adrenal glands are not functioning normally. It can help diagnose congenital adrenal hyperplasia, a genetic condition that affects hormone production, as well as adrenal insufficiency, which occurs when the adrenal glands do not produce enough hormones. The test may also identify other adrenal disorders and help distinguish between different types of adrenal gland problems.

How should I prepare for this test?

Follow your doctor's instructions carefully before the test. Depending on the laboratory's requirements, you may need to fast, meaning that you do not eat or drink anything except water for a specified period. Tell your doctor about every medication, supplement, and herbal product you are taking, because some can affect the results. If necessary, your doctor will advise you to stop certain medicines beforehand. Wear comfortable clothing with sleeves that can easily be rolled up for the blood draw.

What happens during the test?

First, a healthcare professional will inject corticotropin, also called ACTH, into a muscle or a vein. You will then wait for exactly 1 hour before a blood sample is collected from a vein in your arm with a small needle. The blood draw usually takes only a few minutes. You may feel a brief pinch, sting, or scratching sensation when the needle enters your skin. After the procedure, you can generally return to your normal activities unless your healthcare provider gives you different instructions.

Are there any risks?

The risks of this test are generally minimal. You may notice slight pain, bruising, swelling, or minor bleeding at the injection or blood draw site. Some people feel lightheaded during or after the blood draw, particularly if they have not eaten or have had little fluid. The corticotropin injection may temporarily cause facial flushing, an increased heart rate, headache, dizziness, or mild nausea. These effects usually resolve quickly, although serious reactions to the injection are rare.