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Antinuclear Antibody (ANA)

Published: July 27, 2026Updated: August 25, 2026

Antinuclear antibodies (ANA) are a group of autoantibodies that target components within the cell nucleus, often associated with autoimmune conditions.

Quick summary

ANA is a screening biomarker for autoimmune diseases, especially systemic lupus erythematosus (SLE).

Medically reviewed by WellAlly Editorial TeamLast reviewed August 25, 2026Editorial policy

Key facts at a glance

Units
titer
Clinical utility
screening
Result kind
qualitative
FDA-cleared
Roche ANA, Abbott ANA, Siemens ANA, Inova ANA, Zeus ANA
interpretation: {"text":"ANA titer below 1:80; unlikely to indicate an autoimmune condition.","label":"Negative"}; {"text":"ANA titer of 1:80 or higher; may suggest an autoimmune disease, requiring further testing.","label":"Positive"}

Antinuclear antibodies (ANA) are a diverse group of autoantibodies that target various nuclear antigens, such as DNA, histones, and ribonucleoproteins. Their presence is a hallmark of several autoimmune diseases.

The ANA test is commonly used as a first-line screening tool for conditions like systemic lupus erythematosus (SLE), Sjögren's syndrome, and mixed connective tissue disease. A positive result indicates the need for further diagnostic evaluation.

ANA titers are reported as the highest dilution at which fluorescence is still visible. Titers of 1:80 or higher are generally considered positive, but clinical correlation is essential for accurate diagnosis.

Frequently asked questions

What does a positive ANA test mean?
A positive ANA test indicates the presence of antinuclear antibodies, which may suggest an autoimmune disorder. However, further tests are needed for a definitive diagnosis.
Can a healthy person have a positive ANA test?
Yes, a small percentage of healthy individuals may have a positive ANA test, especially at low titers. Clinical correlation is important for interpretation.
  1. 1
    American College of Rheumatology: Antinuclear Antibodies (ANA)Source
  2. 2
    Mayo Clinic: ANA TestSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 25, 2026. · Read our editorial policy