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Progesterone (Mid-Cycle)

Published: July 27, 2026Updated: August 25, 2026

This biomarker measures the level of progesterone in the blood during the mid-cycle phase, which is crucial for assessing ovulation and luteal phase function.

Quick summary

Progesterone mid-cycle levels help diagnose ovulation and luteal phase defects.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 25, 2026Editorial policy

Key facts at a glance

Units
ng/mL
Clinical utility
diagnostic
Result kind
quantitative
FDA-cleared
Roche Progesterone, Abbott Progesterone
interpretation: {"text":"Levels below 3 ng/mL may indicate anovulation or luteal phase defect.","label":"Low"}; {"text":"Levels above 25 ng/mL may suggest overproduction, often due to ovarian cysts or certain medications.","label":"High"}

Progesterone is a hormone produced primarily by the corpus luteum after ovulation. Its levels rise significantly in the mid-luteal phase, typically around day 21 of a 28-day menstrual cycle.

Measuring progesterone during the mid-cycle phase helps determine if ovulation has occurred and if the luteal phase is functioning properly. This is essential for evaluating fertility and hormonal balance.

Abnormal progesterone levels can indicate issues such as anovulation, luteal phase defects, or other hormonal imbalances that may affect fertility and menstrual regularity.

Frequently asked questions

What is a normal progesterone level during the mid-cycle phase?
Normal progesterone levels during the mid-cycle phase range from 3 to 25 ng/mL.
How is a progesterone test performed?
A progesterone test is a simple blood test, usually taken on day 21 of a 28-day menstrual cycle.
  1. 1
    Mayo Clinic: Progesterone TestSource
  2. 2
    American College of Obstetricians and GynecologistsSource
  3. 3
    Progesterone: What It Is, Levels, and MoreSource
  4. 4
    Hormone Health Network: ProgesteroneSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 25, 2026. · Read our editorial policy