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16-Alpha-Hydroxydehydroepiandrosterone (16-OH-DHEA) — Automated Turbidimetric Assay

Published: August 9, 2026Updated: August 17, 2026

This test measures 16-OH-DHEA, a steroid metabolite related to adrenal hormone production, using an automated turbidimetric laboratory method.

Quick summary

["The 16-OH-DHEA test measures a steroid metabolite that may help evaluate adrenal and androgen production."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
110328-2
Short code
16A OH-DHEA Msmt Ur
Synonyms
16A OH-DHEA Msmt Ur
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
135
Ref high
980
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82626
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This blood test measures 16-alpha-hydroxydehydroepiandrosterone, also called 16-OH-DHEA. It is a steroid produced in the body that can provide information about adrenal steroid and androgen production.

Why might I need this test?

A clinician may order this test when symptoms suggest excess androgen activity, an adrenal hormone disorder, or altered steroid metabolism. It may also be used in the evaluation of conditions such as polycystic ovary syndrome or certain rare adrenal disorders.

How is the test performed?

A blood sample is analyzed using an automated turbidimetric method, which estimates the amount of 16-OH-DHEA by measuring how the sample scatters light. The laboratory may use a different validated method, so confirm the details on your report.

How do I prepare for the test?

No special preparation is commonly required, but follow any instructions from the laboratory or clinician. Tell your clinician about steroid medications, hormonal treatments, pregnancy, and recent illness, and do not stop prescribed medication without medical advice.

Reference range

NCBI: DHEA-S Male 135-980 µg/dL; Female 30-260 µg/dLN/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

16-OH-DHEA test

Frequently asked questions

What does 16-OH-DHEA measure?
It measures 16-alpha-hydroxydehydroepiandrosterone, a steroid metabolite formed during adrenal hormone production. The result can provide information about androgen and adrenal steroid activity.
Is this the same as a DHEA-S test?
No. DHEA-S and 16-OH-DHEA are related but distinct substances, and their results are not interchangeable.
Does an abnormal result diagnose a specific disorder?
No. An abnormal result is nonspecific and must be interpreted with symptoms, medical history, other hormone tests, and the laboratory's reference range.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy