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17-Hydroxyandrostenedione and Androstenedione (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures two hormones produced by your adrenal glands and ovaries/testes. They help regulate energy, sex hormones, and metabolism.

Quick summary

["These hormones may affect your adrenal health, hormone balance, and how your body uses energy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
53336-4
Short code
17OHCS+Androst/Cortis DBS
Synonyms
17OHCS+Androst/Cortis DBS
Category
hormone
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend this test to check for conditions like adrenal tumors, hormonal imbalances, or to monitor treatment for conditions like Cushing’s syndrome or congenital adrenal hyperplasia. It could also be part of a broader evaluation for infertility or metabolic disorders.

What the Results Typically Mean

Elevated levels might suggest overactivity of your adrenal glands or certain genetic conditions. Lower-than-expected levels could indicate underactivity or other hormonal issues. Your doctor will interpret results in the context of your overall health and symptoms.

Sample Collection and Preparation

A blood sample is drawn from a vein in your arm. You may feel a slight pinch, but it usually doesn’t cause significant discomfort. The blood is then sent to a lab for testing. No special preparation is needed, but avoid eating or drinking for a few hours before the test if instructed.

Common Conditions Monitored

This test helps diagnose or manage conditions such as adrenal tumors, polycystic ovary syndrome (PCOS), or congenital adrenal hyperplasia. It may also be used to assess hormonal imbalances in people with metabolic disorders or those undergoing fertility treatments.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

17OHCS & Androstenedione

Frequently asked questions

What does a high level of 17-hydroxyandrostenedione or androstenedione mean?
High levels may indicate adrenal tumors, hyperfunction of the adrenal glands, or certain genetic disorders. Your doctor will consider your symptoms, medical history, and other test results to determine the cause and next steps.
What does a low level of these hormones mean?
Low levels might suggest adrenal insufficiency, hormonal deficiencies, or conditions affecting hormone production. This could be due to genetic factors, autoimmune diseases, or other underlying health issues that need evaluation.
How often should I get this test?
The frequency depends on your health condition and treatment plan. Your doctor will decide how often to repeat this test based on your specific situation, symptoms, and response to treatment.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy