Understanding Your Laboratory Test: What to Expect and How to Read Your Results
This test measures the level of 24R-hydroxycalcidiol (a form of vitamin D metabolite) in your blood to help assess vitamin D metabolism and bone health.
{"24R-Hydroxycalcidiol is a breakdown product of vitamin D produced by your kidneys","This test helps evaluate how well your body processes vitamin D","It is often used together with other vitamin D tests to get a complete picture","Abnormal levels may indicate kidney disease or vitamin D deficiency","The test requires a simple blood sample, usually taken in the morning"}
Key facts at a glance
- LOINC
- 1679-0
- Short code
- 24R-OH Calcidiol
- Synonyms
- 24R-OH-Calcidiol SerPl-mCnc
- Category
- chemistry
- Sample
- serum
- Container
- Serum separator tube (SST) or red top tube
- Units
- N/A
- Ref low
- 0.6
- Ref high
- 4.8
- Critical range
- 0.3 N/A – 15 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 18 years
- Method
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
- CPT
- 82310
- SNOMED
- 372687004
- Specimen volume
- 1.0 mL serum minimum, 2.0 mL preferred
- Stability
- Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days. Protect from light.
- Hemolysis max
- 50
- Lipemia max
- 300
- Icterus max
- 20
- TAT
- 72–168 hours
- Order
- routine
This test is a laboratory analysis, usually performed on a small sample of your blood, urine, or other body tissue, that helps your healthcare provider check how well your body is working and look for signs of illness, infection, or other health conditions.
The 24R-hydroxycalcidiol test measures the amount of 24R-hydroxycalcidiol (also called 24R,25-dihydroxyvitamin D3) in your blood. This substance is one of the active forms of vitamin D that your body produces when it processes vitamin D from sunlight, food, or supplements.
This test is important because it gives your doctor objective information about your health, helps confirm or rule out a diagnosis, guides treatment decisions, and can detect potential problems early, often before symptoms become serious or harder to treat.
Vitamin D is essential for maintaining healthy bones and teeth, as it helps your body absorb calcium. The 24R-hydroxycalcidiol test helps doctors understand how well your kidneys are converting vitamin D into its active form. This is particularly useful for evaluating kidney function and bone metabolism.
The test is typically performed by a trained member of our clinical staff. Depending on the type of sample needed, they will draw a small amount of blood from a vein in your arm, ask you to provide a urine sample, or gently swab the affected area. The sample is then sent to the laboratory, and results are usually available within a few days.
A healthcare professional will draw a small sample of blood from a vein in your arm. The sample is then sent to a laboratory for analysis. You do not need to fast before this test, but your doctor may have specific instructions for you.
Your results will show whether your values fall within the normal reference range. Values within range generally suggest no concern in the areas tested, while values that are higher or lower than normal may point to a condition that needs closer attention. Your provider will review the report with you, explain each finding in plain language, and discuss any follow-up testing or treatment if needed.
Results are typically reported in nanograms per milliliter (ng/mL) or another appropriate unit. Your doctor will interpret the results based on your overall health, medical history, and other test results. Low levels may indicate vitamin D deficiency or kidney disease, while elevated levels might suggest excess vitamin D intake or other conditions.
Reference range
Reference range: 0.6-4.8 ng/mL (1.5-12 nmol/L). Values may vary by laboratory methodology and reference standards.N/A
No special preparation required. Fasting is not necessary for this test. Collect sample using standard venipuncture technique. Avoid hemolysis.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings2
Frequently asked questions
Do I need to prepare for this test?
Is the test painful?
How long does it take to get results?
What can cause abnormal 24R-hydroxycalcidiol levels?
Will I need other tests along with this one?
Sources
- 1MedlinePlus - Vitamin DSource
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy