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Understanding Your Laboratory Test: What to Expect and How to Read Your Results

Published: August 9, 2026Updated: August 15, 2026

This test measures the level of 24R-hydroxycalcidiol (a form of vitamin D metabolite) in your blood to help assess vitamin D metabolism and bone health.

Quick summary

{"24R-Hydroxycalcidiol is a breakdown product of vitamin D produced by your kidneys","This test helps evaluate how well your body processes vitamin D","It is often used together with other vitamin D tests to get a complete picture","Abnormal levels may indicate kidney disease or vitamin D deficiency","The test requires a simple blood sample, usually taken in the morning"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
1679-0
Short code
24R-OH Calcidiol
Synonyms
24R-OH-Calcidiol SerPl-mCnc
Category
chemistry
Sample
serum
Container
Serum separator tube (SST) or red top tube
Units
N/A
Ref low
0.6
Ref high
4.8
Critical range
0.3 N/A – 15 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
82310
SNOMED
372687004
Specimen volume
1.0 mL serum minimum, 2.0 mL preferred
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days. Protect from light.
Hemolysis max
50
Lipemia max
300
Icterus max
20
TAT
72–168 hours
Order
routine

This test is a laboratory analysis, usually performed on a small sample of your blood, urine, or other body tissue, that helps your healthcare provider check how well your body is working and look for signs of illness, infection, or other health conditions.

The 24R-hydroxycalcidiol test measures the amount of 24R-hydroxycalcidiol (also called 24R,25-dihydroxyvitamin D3) in your blood. This substance is one of the active forms of vitamin D that your body produces when it processes vitamin D from sunlight, food, or supplements.

This test is important because it gives your doctor objective information about your health, helps confirm or rule out a diagnosis, guides treatment decisions, and can detect potential problems early, often before symptoms become serious or harder to treat.

Vitamin D is essential for maintaining healthy bones and teeth, as it helps your body absorb calcium. The 24R-hydroxycalcidiol test helps doctors understand how well your kidneys are converting vitamin D into its active form. This is particularly useful for evaluating kidney function and bone metabolism.

The test is typically performed by a trained member of our clinical staff. Depending on the type of sample needed, they will draw a small amount of blood from a vein in your arm, ask you to provide a urine sample, or gently swab the affected area. The sample is then sent to the laboratory, and results are usually available within a few days.

A healthcare professional will draw a small sample of blood from a vein in your arm. The sample is then sent to a laboratory for analysis. You do not need to fast before this test, but your doctor may have specific instructions for you.

Your results will show whether your values fall within the normal reference range. Values within range generally suggest no concern in the areas tested, while values that are higher or lower than normal may point to a condition that needs closer attention. Your provider will review the report with you, explain each finding in plain language, and discuss any follow-up testing or treatment if needed.

Results are typically reported in nanograms per milliliter (ng/mL) or another appropriate unit. Your doctor will interpret the results based on your overall health, medical history, and other test results. Low levels may indicate vitamin D deficiency or kidney disease, while elevated levels might suggest excess vitamin D intake or other conditions.

Reference range

Reference range: 0.6-4.8 ng/mL (1.5-12 nmol/L). Values may vary by laboratory methodology and reference standards.N/A

Specimen: serum

No special preparation required. Fasting is not necessary for this test. Collect sample using standard venipuncture technique. Avoid hemolysis.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings2

Frequently asked questions

Do I need to prepare for this test?
Generally, no special preparation is required. However, always follow your doctor's specific instructions. Inform your healthcare provider about any medications or supplements you are taking, as they may affect the results.
Is the test painful?
You may feel a brief pinch or mild discomfort when the blood is drawn, but the pain is usually minimal and temporary.
How long does it take to get results?
Results are typically available within a few days to a week, depending on the laboratory.
What can cause abnormal 24R-hydroxycalcidiol levels?
Low levels may be caused by kidney disease, vitamin D deficiency, liver disease, or certain medications. High levels may result from excessive vitamin D supplementation or certain disorders. Your doctor will consider your complete medical history when interpreting results.
Will I need other tests along with this one?
Yes, doctors often order this test together with other vitamin D tests, such as 25-hydroxyvitamin D, and sometimes calcium and phosphorus tests to get a complete picture of your bone and mineral metabolism.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy