Understanding Your Medical Test: A Comprehensive Guide to What It Is, How It Is Performed, What the Results Mean, and Why You Might Need It
This test detects the presence of 25I-NBOMe, a potent synthetic hallucinogen, in urine samples using confirmatory testing methods.
{"Detects 25I-NBOMe, a synthetic psychedelic drug also known as 'N-bomb' or '25I'","Uses confirmatory testing methods for highly accurate results","May be ordered for emergency department visits, substance abuse monitoring, or forensic investigations","A positive result indicates recent use of this synthetic hallucinogen","Results are confidential and typically shared only with the ordering healthcare provider"}
Key facts at a glance
- LOINC
- 74000-1
- Short code
- 25I-NBOMe U
- Synonyms
- 25I-NBOMe Ur Ql Cfm
- Category
- chemistry
- Sample
- urine
- Container
- Clean, dry, leak-proof plastic urine container with secure lid
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 13–120 years
- Method
- confirm
- CPT
- 80307
- SNOMED
- 373549005
- Specimen volume
- Minimum 10 mL, preferred 20-30 mL
- Stability
- Room temperature: 24-48 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days. Store protected from light.
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 48–168 hours
- Order
- routine
This test is a medical procedure that gathers important information about your health by analyzing a sample taken from your body, such as blood, urine, or tissue. It can help detect the presence of certain diseases, evaluate how well your organs are working, and guide your doctor in making decisions about your care. The test is routine and safe, and the results are used to understand your current medical status or to monitor changes over time.
The 25I-NBOMe urine test is a confirmatory laboratory test that detects the presence of 25I-NBOMe (also called 25I, NBOMe, or 'N-bomb') in urine. 25I-NBOMe is a powerful synthetic hallucinogen belonging to the NBOMe series of substances, which are known for their potent psychedelic effects. This test uses specialized confirmatory methods to accurately identify the drug or its metabolites in the urine sample.
The test is performed by a trained healthcare professional who will collect a sample from you, typically by drawing blood from a vein in your arm, having you provide a urine sample, or taking a small tissue sample with a swab. The collection process is quick and usually causes minimal discomfort. After the sample is collected, it is sent to a laboratory where specialized equipment analyzes it and produces a report for your doctor to review.
A urine sample is collected from the patient, typically in a healthcare setting or laboratory. The sample is then analyzed using confirmatory testing methods, which may include techniques like liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods are highly specific and can accurately detect even small amounts of the drug or its metabolites. The test provides a qualitative result, indicating whether 25I-NBOMe is present or absent in the urine.
The results of this test can indicate whether your values are within the normal range or if there are any abnormalities that may signal a health condition. Your doctor will interpret the results in the context of your medical history, symptoms, and other test results. It is important to understand that abnormal results do not always mean you have a serious problem, and sometimes further testing is needed. Your doctor will explain what the numbers mean for you specifically and discuss next steps.
A 'positive' result means that 25I-NBOMe or its metabolites were detected in the urine, indicating recent use of this synthetic hallucinogen. A 'negative' result means the drug was not detected in the urine at the time of testing. It's important to note that the absence of detection does not necessarily rule out drug use, as the substance may have already been eliminated from the body or concentrations may be below the test's detection threshold.
You might need this test if your doctor wants to screen for a potential health issue, diagnose a suspected condition based on symptoms you are experiencing, monitor the progression of a chronic disease, or check how well a treatment is working. It can also be part of a routine checkup to ensure your body is functioning normally. The test provides critical information that helps your doctor make informed decisions about your health care, so it is often recommended when there is a reason to evaluate your overall well-being.
Healthcare providers may order this test for several reasons: evaluating patients in emergency departments who present with symptoms of drug intoxication or overdose; monitoring individuals in substance abuse treatment programs; conducting forensic investigations; or as part of comprehensive toxicology screening when synthetic drug use is suspected. The test helps healthcare providers make informed decisions about patient care and treatment.
Reference range
Not DetectedN/A
No special preparation required. Provide a clean-catch midstream urine specimen. Avoid first morning sample if possible. Inform the laboratory of any medications or supplements being taken.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Frequently asked questions
How long does 25I-NBOMe stay in urine?
Is this test painful?
Will my employer or insurance company know my results?
Can a positive result be caused by anything other than 25I-NBOMe use?
What happens if my test is positive?
Sources
- 1
- 2
- 3
- 4
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy