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What is this test?

The 25-hydroxyvitamin D test measures the amount of 25-hydroxyvitamin D (also called calcidiol) in your blood. This is the major circulating form of vitamin D and is considered the best indicator of your overall vitamin D status. Vitamin D is essential for maintaining healthy bones and teeth, supporting the immune system, and helping the body absorb calcium.

Why do I need this test?

Your healthcare provider may order this test if you have symptoms of vitamin D deficiency (such as bone pain, muscle weakness, or fatigue) or if you are at risk for vitamin D deficiency. It is also used to monitor treatment with vitamin D supplements and to diagnose conditions like rickets, osteomalacia, or osteoporosis.

How is the test performed?

A healthcare professional will draw a small blood sample from a vein in your arm. The sample is then sent to a laboratory for analysis. No special preparation is usually required, though your doctor may ask you to fast for a certain period if other tests are being done at the same time.

Understanding your results

Results are typically reported in nanograms per milliliter (ng/mL) or nanomoles per liter (nmol/L). Generally, a level of 20-50 ng/mL (50-125 nmol/L) is considered adequate for healthy individuals. Levels below 20 ng/mL (50 nmol/L) may indicate vitamin D deficiency, while levels above 150 ng/mL (375 nmol/L) may indicate vitamin D toxicity.

What can affect my results?

Several factors can influence your vitamin D levels, including: limited sun exposure, darker skin color, older age, certain medical conditions (kidney or liver disease), obesity, certain medications, and dietary intake. Your healthcare provider will interpret your results in the context of your overall health and risk factors.