Skip to content

3meglutaratecsfscnc

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of 3-methylglutarate, an organic acid, in the fluid surrounding the brain and spinal cord to help diagnose certain metabolic disorders.

Quick summary

{"3-Methylglutarate is measured in cerebrospinal fluid (CSF) to detect metabolic disorders","Elevated levels may indicate problems with how the body breaks down certain proteins or fats","This test helps doctors diagnose rare genetic conditions affecting the brain and nervous system","The test requires a lumbar puncture (spinal tap) to collect CSF","Results help distinguish between different types of metabolic diseases"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
72438-5
Short code
3Methylglutarate CSF
Synonyms
3Me-glutarate CSF-sCnc
Category
chemistry
Sample
csf
Container
Sterile polypropylene container for CSF collection
Units
N/A
Ref low
0
Ref high
1
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Gas chromatography-mass spectrometry (GC-MS) or Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
CPT
82491
SNOMED
314432002
Specimen volume
0.5-1.0 mL of cerebrospinal fluid
Stability
CSF should be frozen immediately at -20°C or -80°C if not processed within 30 minutes. Stable for several weeks at -80°C. Avoid repeated freeze-thaw cycles.
Hemolysis max
200
TAT
72–168 hours
Order
routine

What is this test?

The 3-Methylglutarate test measures the amount of a substance called 3-methylglutarate in your cerebrospinal fluid (CSF). CSF is the clear fluid that surrounds and protects your brain and spinal cord. 3-Methylglutarate is an organic acid produced naturally in small amounts when your body breaks down certain amino acids (the building blocks of proteins).

How is this test performed?

A healthcare provider collects CSF through a procedure called a lumbar puncture, also known as a spinal tap. During this procedure, a thin needle is inserted into the lower back to withdraw a small amount of fluid. The sample is then sent to a laboratory where specialists measure the level of 3-methylglutarate using specialized equipment.

Why might I need this test?

Your doctor may order this test if they suspect you or your child has a metabolic disorder. Symptoms that might prompt this test include developmental delays, seizures, muscle weakness, unusual body odors, or episodes of illness that worsen during times of stress or fasting. This test helps doctors determine if there is a problem with how the body processes certain chemicals.

What do the results mean?

Normal results mean that 3-methylglutarate levels are within the expected range. Elevated levels may indicate certain metabolic disorders, such as glutaric acidemia type I, multiple carboxylase deficiency, or other conditions affecting how the body processes proteins. Your doctor will interpret these results along with other tests to make an accurate diagnosis.

Reference range

Reference range for 3-methylglutarate in CSF is typically <1.0 micromol/L (varies by laboratory method and reference population). Results should be interpreted in clinical context with corresponding plasma and urine organic acid profiles.N/A

Specimen: csf

No specific fasting required for CSF collection. Patient should be informed about the lumbar puncture procedure. For metabolic disease investigation, concurrent collection of plasma and urine samples may be recommended for comprehensive diagnostic evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the lumbar puncture procedure painful?
A lumbar puncture is performed with local anesthesia to numb the area, so you may feel pressure but minimal pain. Some people experience a brief headache afterward, which can usually be relieved by lying flat and drinking fluids.
How long does it take to get results?
This specialized test is sent to specialized laboratories that perform organic acid analysis. Results typically take several days to a week, as the analysis requires specialized equipment and expertise.
Do I need to fast before this test?
Follow your doctor's instructions regarding eating and drinking before the test. In some cases, fasting may be required, as certain foods can affect metabolic markers. Your healthcare provider will give you specific instructions.
Can this test diagnose all metabolic disorders?
No, this test specifically measures 3-methylglutarate. Your doctor may order additional tests to check for other organic acids or metabolic markers to get a complete picture of your condition.
  1. 1
    MedlinePlus - Lumbar Puncture (Spinal Tap)Source
  2. 2
    National Library of Medicine - Organic Acid AnalysisSource
  3. 3
    Mayo Clinic Laboratories - Organic Acids PanelSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy