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3mehistidineamnscnc

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of 3-methylhistidine in amniotic fluid to assess fetal muscle development and protein metabolism.

Quick summary

{"3-Methylhistidine is a marker of muscle protein breakdown","This test helps evaluate fetal muscle development during pregnancy","It is particularly useful in assessing fetal growth restriction","The test is performed on amniotic fluid collected during amniocentesis","Results are compared to normal ranges for gestational age"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27176-7
Short code
3Me-histidine Amn-sCnc
Synonyms
3Me-histidine Amn-sCnc
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

What is this test?

The 3-methylhistidine test measures the amount of 3-methylhistidine in the amniotic fluid that surrounds the baby during pregnancy. 3-Methylhistidine is a compound produced when muscle proteins break down in the body. By measuring its levels in amniotic fluid, healthcare providers can gain insights into the baby's muscle development and overall protein metabolism.

How is the test performed?

This test requires a sample of amniotic fluid, which is collected through a procedure called amniocentesis. During amniocentesis, a thin needle is used to withdraw a small amount of amniotic fluid from the uterus under ultrasound guidance. The fluid sample is then sent to a laboratory for analysis. The procedure is generally safe but carries a small risk of complications such as infection or miscarriage, which is why it is typically performed only when medically necessary.

Why is this test important?

Measuring 3-methylhistidine in amniotic fluid provides valuable information about fetal muscle development. This is particularly important in cases where there are concerns about fetal growth restriction or when a healthcare provider needs to evaluate whether the baby is developing muscles properly. The test helps doctors assess whether the fetus is receiving adequate nutrition and whether muscle tissue is forming as expected during pregnancy.

What do the results mean?

Results are interpreted based on the gestational age of the pregnancy. Abnormal levels of 3-methylhistidine may indicate issues with fetal muscle development or protein metabolism. Your healthcare provider will explain your specific results and what they mean for your pregnancy. It is important to remember that this test is just one tool among many used to assess fetal health, and results are always considered alongside other clinical findings and test results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the amniocentesis procedure painful?
Most women experience some discomfort during amniocentesis, similar to a menstrual cramp. The procedure is performed under ultrasound guidance, and a local anesthetic may be used to minimize pain. The entire procedure usually takes about 10-15 minutes.
What are the risks of amniocentesis?
Amniocentesis carries a small risk of complications, including infection, bleeding, leakage of amniotic fluid, and a very small risk of miscarriage (approximately 1 in 300 to 1 in 500). Your healthcare provider will discuss these risks with you before the procedure.
How long does it take to get results?
Results from the 3-methylhistidine test typically take several days to a week, as the analysis requires specialized laboratory testing. Your healthcare provider will contact you with the results as soon as they are available.
Will I need to have this test repeated?
Whether you need repeat testing depends on your specific clinical situation. Your healthcare provider will determine if additional testing is necessary based on the initial results, your pregnancy progress, and other clinical factors.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy