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5-Alpha-Tetrahydrocortisol, 24-Hour Urine — Excretion Rate

Published: August 9, 2026Updated: August 16, 2026

A 24-hour urine test measuring excretion of 5-alpha-tetrahydrocortisol, a metabolite of cortisol, to help assess adrenal steroid production.

Quick summary

["This test measures how much cortisol metabolite is released in the urine over 24 hours and may help evaluate conditions involving excess cortisol."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
73862-5
Short code
5-A THF 24h Ur-sRate
Synonyms
5-A THF 24h Ur-sRate
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of 5-alpha-tetrahydrocortisol, a cortisol metabolite, excreted in urine during a 24-hour period. It provides information about the body’s production and metabolism of cortisol.

Why might I need this test?

It may be ordered when symptoms suggest excess cortisol production or Cushing syndrome. The result is usually interpreted with other cortisol and adrenal steroid tests.

How is the test performed?

You collect all urine for exactly 24 hours, usually in a large sterile container provided by the laboratory. The collection start time, end time, and any missed samples should be recorded.

How do I prepare for the test?

Follow the laboratory’s collection instructions and note the date and time that the collection begins. Refrigerate the container if instructed, do not flush it down the toilet, and return the full sample promptly.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine 5-Alpha-Tetrahydrocortisol

Frequently asked questions

What does this test measure?
It measures the 24-hour urinary excretion of 5-alpha-tetrahydrocortisol, which is a metabolite formed when the body breaks down cortisol.
How is a 24-hour urine collection done?
Collect all urine into the designated container for 24 hours, including urine produced at the end of the collection period. Record the start and end times and tell the laboratory if any urine was missed.
Does one abnormal result confirm Cushing syndrome?
No. This test is one part of the evaluation and is usually interpreted alongside other cortisol measurements and clinical findings.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy