Skip to content

5-Alpha-Tetrahydrodeoxycortisol 2,3-Diol (High Deoxycortisol), 24-Hour Urine — Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

A 24-hour urine test that measures a steroid metabolite associated with adrenal hormone production and certain adrenal enzyme disorders.

Quick summary

["This test helps clinicians evaluate abnormal adrenal steroid production, particularly when congenital adrenal hyperplasia is suspected."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57615-7
Short code
5-A THF 2D p high Dex 24h Ur-mCnc
Synonyms
5-A THF 2D p high Dex 24h Ur-mCnc
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures 5-alpha-tetrahydrodeoxycortisol 2,3-diol, also described as a high deoxycortisol metabolite, in urine collected over 24 hours. It is a specialized adrenal steroid test used to evaluate the body's production of cortisol and related hormones.

Why might I need this test?

A clinician may order this test when there is concern for congenital adrenal hyperplasia, an inherited disorder that affects adrenal hormone production. It may also be used alongside other steroid tests, symptoms, and physical findings to help identify the type of enzyme problem involved.

How is the test performed?

You collect all urine for a full 24-hour period, usually beginning in the morning and ending the following morning. The laboratory measures the amount of this metabolite in the collection, and the reported result may also account for the total urine volume.

How do I prepare for the test?

Follow the collection instructions provided by the laboratory and record the collection time and total urine volume. Keep the container refrigerated during collection if instructed, and tell the healthcare team about corticosteroid or other steroid medicines; do not stop prescribed medication without medical advice.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

5-Alpha-THF 2,3-Diol, 24-Hour Urine

Frequently asked questions

Why is a 24-hour urine collection required?
Measuring the metabolite in all urine collected over 24 hours provides a more representative estimate of daily steroid production than a single urine or blood sample.
Does an abnormal result mean I definitely have an adrenal disorder?
No. This test is interpreted with symptoms, examination findings, and other adrenal hormone measurements because medications and collection problems can also affect the result.
What if I miss part of the urine collection?
An incomplete collection can make the result less reliable. Contact the laboratory or ordering clinician for guidance on whether the collection needs to be repeated.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy