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5ntamnccnc

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of 5'-nucleotidase enzyme in amniotic fluid to assess fetal liver and biliary system development.

Quick summary

{"5'-Nucleotidase is an enzyme produced by fetal liver and biliary tract","This test helps evaluate if the fetal liver and bile ducts are developing normally","Abnormal levels may indicate liver disease or bile duct problems in the fetus","The test is performed on amniotic fluid collected during amniocentesis","Results are often combined with other tests for a complete assessment"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
1689-9
Short code
5NT Amn-cCnc
Synonyms
5NT Amn-cCnc
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

What is this test?

The 5'-Nucleotidase test measures the activity of an enzyme called 5'-nucleotidase (5'-NT) in the amniotic fluid that surrounds the unborn baby (fetus). 5'-Nucleotidase is primarily produced by the fetal liver and biliary system (the tubes that carry bile from the liver).

How is the test performed?

This test requires a sample of amniotic fluid, which is collected through a procedure called amniocentesis. During amniocentesis, a thin needle is inserted through the mother's abdomen into the uterus to withdraw a small amount of amniotic fluid. The fluid sample is then sent to a laboratory for analysis.

Why is it important?

Measuring 5'-nucleotidase in amniotic fluid helps doctors evaluate whether the fetal liver and biliary system are developing properly. This enzyme is normally present in amniotic fluid at certain levels due to normal fetal development and metabolism.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is this test safe?
Amniocentesis carries a small risk of complications such as infection, bleeding, or miscarriage. Your healthcare provider will discuss the risks and benefits with you. The test itself is performed under ultrasound guidance to minimize risks.
When is this test typically ordered?
This test may be recommended when there are concerns about fetal liver or biliary development, such as suspected biliary atresia or other liver disorders. It may also be part of a comprehensive evaluation if abnormalities are detected on ultrasound.
How long does it take to get results?
Results typically take several days to a week, as the test requires specialized laboratory analysis. Your doctor will discuss the results with you once they are available.
Will I need additional tests?
Yes, 5'-nucleotidase results are often interpreted alongside other tests, including other liver enzymes in amniotic fluid and possibly additional imaging studies, to make a complete assessment of fetal health.
  1. 1
    Mayo Clinic Laboratories - Test CatalogSource
  2. 2
    ARUP Laboratories - Clinical EncyclopediaSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy