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6-Alpha-Hydroxytestosterone, 24-Hour Urine — Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures 6-alpha-hydroxytestosterone in urine collected over 24 hours to help evaluate adrenal steroid production.

Quick summary

["This specialized urine test can help identify changes in adrenal steroid production when interpreted with other clinical and laboratory findings."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57601-7
Short code
6A-OH-THS Pre Dex 24h Ur-mCnc
Synonyms
6A-OH-THS Pre Dex 24h Ur-mCnc
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

6-alpha-hydroxytestosterone is a steroid-related substance. This test measures its amount in a urine sample collected over 24 hours and is used as a specialized marker of adrenal steroid metabolism.

Why might I need this test?

This test may be ordered when a clinician is evaluating an adrenal hormone disorder, unusual androgen or estrogen production, or symptoms related to a congenital adrenal enzyme deficiency. It is usually interpreted together with other hormone tests rather than by itself.

How is the test performed?

You collect all urine for 24 hours using the container and instructions supplied by the laboratory. The laboratory then measures the concentration of 6-alpha-hydroxytestosterone in the collected specimen.

How do I prepare for the test?

Follow the laboratory's 24-hour collection instructions carefully, including any storage requirements. If a collection is missed, contact the laboratory for guidance, and do not stop medications unless your healthcare professional instructs you to do so.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

6-Alpha-Hydroxytestosterone, 24-Hour Urine

Frequently asked questions

What does this test measure?
It measures the concentration of 6-alpha-hydroxytestosterone, a steroid-related substance, in a 24-hour urine collection.
Why is a 24-hour urine collection used?
Collecting urine for the full day helps reduce the effect of temporary fluctuations and provides a more representative measurement for evaluation.
Does an abnormal result diagnose an adrenal disorder?
No. The result is one part of the evaluation and must be interpreted alongside other laboratory tests, medical history, and examination findings.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy