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Alanine Aminotransferase (ALT) Test

Published: August 9, 2026Updated: August 17, 2026

Measures alanine aminotransferase enzyme activity to help evaluate liver injury and other causes of elevated liver enzymes.

Quick summary

["An elevated ALT result may indicate liver injury, but it must be interpreted with your symptoms, medications, and other test results."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
51540-3
Short code
A alternata IgG Ql
Synonyms
A alternata IgG Ql
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
7
Ref high
56
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84484
SNOMED
302773008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

Alanine aminotransferase, commonly called ALT, is an enzyme found in liver cells and, to a lesser extent, in other organs such as the heart and skeletal muscle. The test measures ALT enzyme activity and is mainly used to evaluate possible liver injury.

Why might I need this test?

ALT may be checked during routine health examinations or when a clinician is evaluating abnormal liver-related symptoms, medication effects, alcohol use, or other conditions that could affect the liver.

How is the test performed?

The abbreviated code does not clearly identify the specimen or assay method. ALT is generally measured in serum or plasma using an automated enzymatic assay, which measures the rate at which the enzyme acts on a laboratory substrate.

How do I prepare for the test?

Fasting is usually not required for a routine ALT test, but follow the instructions provided by your clinician or laboratory. Tell the healthcare team about all medications and supplements you use, and avoid unusually heavy alcohol consumption or strenuous exercise before testing if possible because these can affect the result.

Reference range

Adult males: 7-56 U/L; Adult females: 7-43 U/LN/A

Specimen: serum

No special preparation required. Avoid fatty meals before the test if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ALT

Frequently asked questions

Is ALT the same as AST?
No. ALT and aspartate aminotransferase, or AST, are different enzymes that can both rise when tissues are injured. A clinician may compare them to help determine the likely source of an elevated result.
Do I need to fast before an ALT test?
Fasting is usually not necessary unless your clinician specifically requests it. If the test was ordered as part of a broader panel, follow the preparation instructions for that panel.
What should I do if my ALT is elevated?
Discuss the result with your healthcare professional rather than trying to treat it on your own. The result may lead to repeat testing, review of medications and alcohol use, or additional liver tests.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy