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Acepromazineurmcnc

Published: August 9, 2026Updated: August 16, 2026

This test detects acepromazine, a sedative medication used in animals, in urine samples.

Quick summary

{"Acepromazine is a sedative drug commonly used in horses and other animals","This urine test checks if an animal has been exposed to acepromazine","The test is often used in equine sports to ensure fair competition","Results help veterinarians and trainers understand recent medication use","Detection windows vary depending on dose and individual animal metabolism"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
60044-5
Short code
ACE U
Synonyms
Acepromazine Ur-mCnc
Category
chemistry
Sample
urine
Container
Clean, sterile, leak-proof plastic urine container without preservatives
Units
N/A
Ref low
0
Ref high
10
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) or Gas chromatography-mass spectrometry (GC-MS)
CPT
80307 (Drug testing, per specimen, qualitative or quantitative)
SNOMED
39873001 (Urine specimen)
Specimen volume
Minimum 5 mL; preferred 10-15 mL for confirmatory testing
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this test?

The Acepromazine urine test is a laboratory analysis that detects the presence of acepromazine, a tranquilizer medication, in a urine sample. Acepromazine (also called acetylpromazine or ACE) is primarily used in veterinary medicine as a sedative and pre-anesthetic medication, most commonly in horses but also in other animals like dogs and cats.

How is the test performed?

A urine sample is collected from the animal and sent to a laboratory for analysis. The laboratory uses specialized equipment to detect and measure the amount of acepromazine or its metabolites present in the urine. The test results are typically reported as either positive (present) or negative (not detected), or may include quantitative levels if requested.

Why is this test ordered?

This test may be ordered for several reasons: (1) In equine competitions or racing to ensure horses have not been illegally administered sedatives before events, (2) In veterinary hospitals to monitor medication use and ensure proper withdrawal times before competition, (3) In cases of unexplained sedation or behavior changes in animals, or (4) As part of a comprehensive drug screening panel for performance animals.

Reference range

Not detected or < 10 ng/mL (screening cutoff); Confirmatory testing performed if initial result is above cutoffN/A

Specimen: urine

Collect clean-catch midstream urine sample. Avoid contamination with toilet water or cleansing agents. Inform laboratory of any medications or supplements being taken.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long does acepromazine stay detectable in urine?
Acepromazine can typically be detected in urine for 48-72 hours after administration, though this can vary based on the dose given, the individual animal's metabolism, and the sensitivity of the testing method used.
Is acepromazine safe for animals?
When used appropriately under veterinary supervision, acepromazine is generally safe. However, it can have side effects including lowered blood pressure, excessive drooling, and in rare cases, penile prolapse in male horses. It should only be used as directed by a licensed veterinarian.
Why is this test important for horse racing?
In competitive equestrian sports and racing, horses must compete without any performance-altering substances. Acepromazine can calm nervous horses, potentially improving their performance. Therefore, most racing and competitive organizations prohibit its use close to events, and testing ensures fair competition.
What happens if the test is positive?
If acepromazine is detected in a competition animal, it may result in disqualification from the event. The specific consequences depend on the competition rules and regulations of the governing organization. For non-competition animals, a positive result simply indicates recent medication administration.
  1. 1
    LOINC Code ReferenceSource
  2. 2
    American Association of Equine Practitioners - Acepromazine GuidelinesSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy