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Acetazolamideserplscnc

Published: August 9, 2026Updated: August 10, 2026

This test measures the amount of acetazolamide in your blood to ensure the drug is at the right level for effective treatment.

Quick summary

{"Measures acetazolamide concentration in blood serum","Helps doctors ensure the drug dose is correct and safe","Blood sample is typically collected after 3-5 days of consistent dosing","Therapeutic monitoring helps prevent side effects and ensure efficacy","Results help guide personalized dosing adjustments"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 10, 2026Editorial policy

Key facts at a glance

LOINC
55522-7
Short code
ACETAM
Synonyms
acetaZOLAMIDE SerPl-sCnc
Category
chemistry
Sample
serum
Container
Serum separator tube (SST) or plain red top tube; plasma in EDTA or heparin tube acceptable
Units
N/A
Ref low
5
Ref high
15
Critical range
— – 30 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
High Performance Liquid Chromatography (HPLC) or Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
80375
Specimen volume
2-3 mL serum or plasma minimum
Stability
Room temperature: 7 days; Refrigerated (2-8°C): 14 days; Frozen (-20°C or below): 30 days. Protect from light.
Hemolysis max
50
Lipemia max
300
Icterus max
60
TAT
24–72 hours
Order
routine

What is this test?

The Acetazolamide blood level test is a laboratory test that measures the concentration of acetazolamide in your serum or plasma. Acetazolamide is a medication called a carbonic anhydrase inhibitor, used to treat conditions such as glaucoma, epilepsy, altitude sickness, and certain types of fluid retention.

Why do I need this test?

Your doctor may order this test to: (1) Monitor that the drug level in your blood is within the therapeutic range, (2) Ensure you are receiving an effective dose, (3) Check for signs of toxicity or overdose, (4) Adjust your dose based on how your body processes the medication.

How is the test performed?

A healthcare professional will draw a blood sample from a vein in your arm. The blood will be collected in a tube and sent to a laboratory for analysis. The test is usually performed when the drug has reached steady state, typically 3-5 days after starting treatment or after any dose change.

What should I do to prepare?

Follow your doctor's instructions regarding taking your acetazolamide dose. Usually, the blood sample is collected just before your next dose (trough level) to ensure accurate measurement. Inform your doctor about all medications and supplements you are taking.

Are there any risks?

The risks associated with blood drawing are minimal and may include slight pain, bruising, or bleeding at the puncture site. Some people may feel lightheaded.

Reference range

Therapeutic range: 5-15 mcg/mL (trough levels); 10-25 mcg/mL (peak levels). Therapeutic range in SI units: 23-70 umol/L (trough); 46-116 umol/L (peak).N/A

Specimen: serum

Collect as trough level immediately before next dose. No fasting required. Inform laboratory of dose timing and last administration. Record medication dosage and time of last dose on requisition.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

What is a therapeutic range for acetazolamide?
The therapeutic range for acetazolamide is typically between 5-15 mcg/mL (micrograms per milliliter). However, the exact target range may vary slightly between laboratories and your doctor will interpret your results based on your specific condition and treatment goals.
How often do I need this test?
The frequency of testing depends on your individual situation. Your doctor may order this test when you first start treatment, after any dose change, if you experience symptoms of side effects, or if you have conditions that affect drug metabolism (such as kidney or liver disease).
What happens if my levels are too high?
If your acetazolamide level is above the therapeutic range, you may be at risk for side effects such as drowsiness, confusion, tingling sensations, loss of appetite, or kidney stones. Your doctor may lower your dose or switch to a different medication.
What happens if my levels are too low?
If your level is below the therapeutic range, the medication may not be effectively controlling your condition. Your doctor may consider increasing your dose or exploring alternative treatment options.
Can I take my acetazolamide before the blood test?
Follow your doctor's specific instructions. Typically, for a trough level (lowest point) measurement, blood is drawn just before your next scheduled dose. Always confirm with your healthcare provider about taking your medication on the day of the test.
  1. 1
    MedlinePlus - AcetazolamideSource
  2. 2
    Mayo Clinic Laboratories - Therapeutic Drug MonitoringSource
  3. 3
    LOINC - Acetazolamide [Moles/volume] in Serum or PlasmaSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 10, 2026. · Read our editorial policy