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Acetoacetmsmtur

Published: August 9, 2026Updated: August 16, 2026

A urine test that measures acetoacetate levels to help diagnose and monitor conditions like diabetic ketoacidosis.

Quick summary

{"Acetoacetate is a type of ketone body produced when the body breaks down fat for energy","This test is primarily used to check for diabetic ketoacidosis (DKA)","It can also monitor ketone levels during fasting, low-carb diets, or alcohol withdrawal","Results are reported as positive or negative, indicating whether ketones are present in urine","High ketone levels in urine may require immediate medical attention"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
108593-5
Short code
ACETU
Synonyms
Acetoacet Msmt Ur
Category
chemistry
Sample
urine
Container
Sterile urine container (clean-catch or random specimen container)
Units
N/A
Ref low
0
Ref high
1
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Enzymatic method using beta-hydroxybutyrate dehydrogenase or chemical method using nitroprusside reaction
CPT
80378
SNOMED
271544004
Specimen volume
10-50 mL preferred
Stability
Refrigerate at 2-8°C if not processed within 2 hours; stable for up to 24 hours refrigerated; freeze at -20°C for longer storage
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
1–24 hours
Order
routine

What is this test?

The urine acetoacetate test measures the amount of acetoacetate, a type of ketone body, in your urine. Ketones are produced when your body breaks down fat for energy instead of using glucose. This typically happens when there is not enough insulin to use glucose for energy, such as in diabetes, or during prolonged fasting, very low-carbohydrate diets, or excessive alcohol consumption.

Why is this test performed?

This test is mainly ordered to: 1) Diagnose and monitor diabetic ketoacidosis (DKA), a serious complication of diabetes; 2) Check ketone levels in people with diabetes, especially when blood sugar is consistently high; 3) Monitor individuals following ketogenic diets or fasting protocols; 4) Evaluate unexplained symptoms like nausea, vomiting, or abdominal pain that may be related to ketone buildup.

How is the test done?

A urine sample is collected in a sterile container. You may be asked to clean the genital area before collection to avoid contamination. The sample is then sent to a laboratory for analysis. Some healthcare providers may use dipstick tests that provide immediate results, though laboratory testing is more accurate.

What do the results mean?

Results are typically reported as negative (normal) or positive. A negative result means no detectable ketones in the urine, which is normal. A positive result indicates ketones are present and is usually reported as small (+), moderate (++), or large (+++). Higher levels suggest more significant ketone production, which may indicate diabetic ketoacidosis or other metabolic conditions requiring prompt medical attention.

Reference range

Negative or undetectable (< 1 mg/dL); trace amounts may be considered normal in fasting statesN/A

Specimen: urine

No special preparation required; first morning urine specimen preferred for optimal accuracy; inform physician of any medications or supplements

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is fasting required before this test?
No specific fasting is required. However, your doctor may ask you to avoid certain foods or supplements before the test. Inform your healthcare provider about any medications or supplements you are taking, as some may affect test results.
Can I do this test at home?
Yes, over-the-counter urine ketone test strips are available for home use. These dipstick tests can detect acetoacetate in urine. However, home testing should not replace laboratory testing, especially if you have diabetes or suspect DKA. Always discuss results with your healthcare provider.
What happens if my test shows high ketones?
If your test shows moderate to high ketone levels, contact your healthcare provider immediately, especially if you have diabetes. High ketones can lead to diabetic ketoacidosis, which requires prompt medical treatment. Symptoms like excessive thirst, frequent urination, nausea, vomiting, abdominal pain, or confusion should prompt immediate medical attention.
Are there any risks associated with this test?
The urine acetoacetate test is very safe with minimal risks. You may experience mild discomfort during urine collection. There is a very small risk of urinary tract infection from the collection process, but this is uncommon when proper cleaning techniques are used.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy