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Understanding Your Laboratory Test: What Patients Need to Know

Published: August 9, 2026Updated: August 10, 2026

A simple blood test to detect the presence of acetone, a type of ketone body, in your serum.

Quick summary

{"Measures whether acetone (a ketone) is present in your blood","Helps diagnose and monitor diabetic ketoacidosis (DKA)","A positive result means ketones are found in your blood","Sample is collected from a vein in your arm","Often ordered along with other tests like glucose and electrolytes"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 10, 2026Editorial policy

Key facts at a glance

LOINC
16310-5
Short code
ACET
Synonyms
Acetone Fld Ql
Category
chemistry
Sample
serum
Container
Serum separator tube (SST) or plain red top tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–120 years
Method
Enzymatic (beta-hydroxybutyrate dehydrogenase) or Chemical (Nitroprusside reaction)
CPT
80378
SNOMED
2736001
Specimen volume
1-2 mL serum minimum
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
Hemolysis max
50
Lipemia max
100
Icterus max
20
TAT
2–24 hours
Order
routine

This laboratory test measures specific substances in a sample of your blood, urine, or other body fluid to help your healthcare provider evaluate how your body is working. It may be used to screen for a medical condition, help diagnose symptoms you are experiencing, or monitor a condition or treatment you already have.

The acetone presence test is a qualitative test that checks whether acetone, a type of ketone body, is present in your blood serum. Normally, your body uses glucose for energy. When there is not enough insulin (like in diabetes) or when you are not eating enough carbohydrates (like in prolonged fasting), your body breaks down fat instead, producing ketones including acetone.

The test is usually performed by collecting a small sample in a clinic, hospital, or laboratory. A trained nurse or phlebotomist will clean the site, draw blood from a vein in your arm, or ask you to provide a urine sample, and then send it to a certified laboratory for analysis. The procedure is quick, and most people can return to their normal activities right away.

A healthcare professional will draw a small blood sample from a vein in your arm using a needle. The blood is collected in a tube and sent to the laboratory for analysis. You do not need to fast for this test unless your doctor instructs otherwise.

Your results are compared with a normal reference range for your age and sex. Results within the normal range generally suggest no evidence of the condition being tested, while abnormal values may point to infection, inflammation, organ dysfunction, or another issue that requires follow-up. Always discuss your results with your doctor, who can explain what they mean for your overall health.

The test result is reported as either 'Positive' (acetone is present) or 'Negative' (acetone is not detected). A positive result indicates that your body is producing excess ketones, which may require medical attention. A negative result means no acetone was detected in your blood at the time of testing.

Reference range

Negative/Not detectedN/A

Specimen: serum

No special preparation required. Inform laboratory of any medications, supplements, or recent alcohol consumption that may affect results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Detecting acetone in blood is primarily used to help diagnose and monitor diabetic ketoacidosis (DKA), a serious complication of diabetes where high blood sugar and ketone buildup lead to dangerous acidity in the blood. This test may also be useful in evaluating patients with unexplained metabolic acidosis, prolonged fasting, or certain metabolic disorders. Results are typically interpreted alongside other laboratory values such as blood glucose, electrolytes, and arterial blood gas measurements.

Frequently asked questions

Do I need to fast before this test?
Fasting is not typically required for the acetone presence test. However, follow any specific instructions given by your healthcare provider.
Is this test painful?
You may feel a brief sting or pinch when the needle is inserted to draw blood. This is generally well-tolerated and the procedure only takes a few minutes.
What does a positive result mean?
A positive result means acetone was detected in your blood. This could indicate diabetic ketoacidosis, prolonged fasting, a very low-carbohydrate diet, or other metabolic conditions. Your doctor will evaluate this result along with other tests to determine the cause and appropriate treatment.
How is this different from a breath acetone test?
This test measures acetone in your blood (serum), while breath acetone tests measure acetone in exhaled air. Blood testing is generally more accurate and is the standard method used in medical settings.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 10, 2026. · Read our editorial policy