Understanding Your Laboratory Test: What Patients Need to Know
A simple blood test to detect the presence of acetone, a type of ketone body, in your serum.
{"Measures whether acetone (a ketone) is present in your blood","Helps diagnose and monitor diabetic ketoacidosis (DKA)","A positive result means ketones are found in your blood","Sample is collected from a vein in your arm","Often ordered along with other tests like glucose and electrolytes"}
Key facts at a glance
- LOINC
- 16310-5
- Short code
- ACET
- Synonyms
- Acetone Fld Ql
- Category
- chemistry
- Sample
- serum
- Container
- Serum separator tube (SST) or plain red top tube
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–120 years
- Method
- Enzymatic (beta-hydroxybutyrate dehydrogenase) or Chemical (Nitroprusside reaction)
- CPT
- 80378
- SNOMED
- 2736001
- Specimen volume
- 1-2 mL serum minimum
- Stability
- Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
- Hemolysis max
- 50
- Lipemia max
- 100
- Icterus max
- 20
- TAT
- 2–24 hours
- Order
- routine
This laboratory test measures specific substances in a sample of your blood, urine, or other body fluid to help your healthcare provider evaluate how your body is working. It may be used to screen for a medical condition, help diagnose symptoms you are experiencing, or monitor a condition or treatment you already have.
The acetone presence test is a qualitative test that checks whether acetone, a type of ketone body, is present in your blood serum. Normally, your body uses glucose for energy. When there is not enough insulin (like in diabetes) or when you are not eating enough carbohydrates (like in prolonged fasting), your body breaks down fat instead, producing ketones including acetone.
The test is usually performed by collecting a small sample in a clinic, hospital, or laboratory. A trained nurse or phlebotomist will clean the site, draw blood from a vein in your arm, or ask you to provide a urine sample, and then send it to a certified laboratory for analysis. The procedure is quick, and most people can return to their normal activities right away.
A healthcare professional will draw a small blood sample from a vein in your arm using a needle. The blood is collected in a tube and sent to the laboratory for analysis. You do not need to fast for this test unless your doctor instructs otherwise.
Your results are compared with a normal reference range for your age and sex. Results within the normal range generally suggest no evidence of the condition being tested, while abnormal values may point to infection, inflammation, organ dysfunction, or another issue that requires follow-up. Always discuss your results with your doctor, who can explain what they mean for your overall health.
The test result is reported as either 'Positive' (acetone is present) or 'Negative' (acetone is not detected). A positive result indicates that your body is producing excess ketones, which may require medical attention. A negative result means no acetone was detected in your blood at the time of testing.
Reference range
Negative/Not detectedN/A
No special preparation required. Inform laboratory of any medications, supplements, or recent alcohol consumption that may affect results.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Detecting acetone in blood is primarily used to help diagnose and monitor diabetic ketoacidosis (DKA), a serious complication of diabetes where high blood sugar and ketone buildup lead to dangerous acidity in the blood. This test may also be useful in evaluating patients with unexplained metabolic acidosis, prolonged fasting, or certain metabolic disorders. Results are typically interpreted alongside other laboratory values such as blood glucose, electrolytes, and arterial blood gas measurements.
Frequently asked questions
Do I need to fast before this test?
Is this test painful?
What does a positive result mean?
How is this different from a breath acetone test?
Sources
- 1
- 2MedlinePlus - KetonesSource
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 10, 2026. · Read our editorial policy