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Acetylcarnsmnscnc

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of acetylcarnitine in semen, which is important for evaluating male fertility and sperm function.

Quick summary

{"Acetylcarnitine is a key energy source for sperm cells","Low levels may indicate male fertility problems","The test helps doctors assess sperm quality and function","It is often part of a comprehensive fertility evaluation","Results help guide treatment decisions for infertility"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
54345-4
Short code
C2
Synonyms
Acetylcarn Smn-sCnc
Category
chemistry
Sample
serum
Container
Sterile, wide-mouthed plastic specimen container (without preservatives or lubricants)
Units
N/A
Ref low
0.8
Ref high
2.5
Fasting
false
Population
adult
Sex
male
Age range
18–65 years
Method
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) or High-performance liquid chromatography (HPLC) with fluorescence detection
CPT
82379
SNOMED
308441009
Specimen volume
1-2 mL of semen (entire ejaculate preferred for accurate measurement)
Stability
Room temperature: 30-60 minutes; Refrigerated (2-8°C): up to 24 hours; Frozen (-20°C or below): several weeks (avoid repeated freeze-thaw cycles)
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–72 hours
Order
routine

What is this test?

The acetylcarnitine (C2) test measures the concentration of acetylcarnitine in your semen. Acetylcarnitine is a molecule that plays a crucial role in energy metabolism, particularly in sperm cells. It helps sperm cells generate the energy they need for movement and function.

Why is it important?

Sperm cells require significant energy to swim and fertilize an egg. Acetylcarnitine serves as an important fuel source for mitochondria in sperm cells, supporting their motility and overall function. Adequate levels of acetylcarnitine in semen are associated with healthy sperm parameters.

How is the test performed?

You will need to provide a semen sample, usually collected through masturbation after a period of sexual abstinence (typically 2-5 days). The sample is then analyzed in a laboratory using specialized equipment to measure the concentration of acetylcarnitine.

What do the results mean?

Normal acetylcarnitine levels in semen indicate good sperm energy metabolism and function. Low levels may suggest problems with sperm quality or quantity, which could contribute to infertility. Your doctor will interpret your results along with other fertility tests to assess your reproductive health.

Reference range

Reference range: 0.8-2.5 µmol/L (typical range; laboratory-specific reference ranges apply)N/A

Specimen: serum

Maintain sexual abstinence for 2-5 days before collection. Do not use lubricants, condoms, or saliva during collection. Collect specimen via masturbation into a sterile, wide-mouthed container. Deliver to laboratory within 60 minutes of collection. Keep specimen at body temperature during transport.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Do I need to prepare for the test?
Your doctor may recommend abstaining from sexual activity for 2-5 days before providing the sample. You should also avoid alcohol, caffeine, and smoking for a few days before the test, as these can affect semen quality. Follow any specific instructions provided by your healthcare provider.
Is the test painful?
No, the test is not painful. You will provide a semen sample through masturbation in a private room at the clinic or laboratory. The sample collection process is similar to a routine semen analysis.
How long does it take to get results?
Results are typically available within a few days to a week, depending on the laboratory. Your doctor will discuss the results with you during a follow-up appointment.
What can I do if my levels are low?
If your acetylcarnitine levels are low, your doctor may recommend lifestyle changes such as maintaining a healthy weight, quitting smoking, reducing alcohol consumption, and managing stress. In some cases, supplementation with L-carnitine or acetyl-L-carnitine may be suggested. Your doctor will create a personalized treatment plan based on your specific situation.
Can this test diagnose the cause of infertility?
This test helps evaluate sperm function but does not diagnose the specific cause of infertility. It is usually part of a comprehensive fertility evaluation that includes other tests such as semen analysis, hormone testing, and physical examination. Your doctor will use all this information to determine the likely cause and appropriate treatment options.
  1. 1
    Mayo Clinic - Male InfertilitySource
  2. 2
    MedlinePlus - Semen AnalysisSource
  3. 3
    American Society for Reproductive MedicineSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy