Acth25hpgcplasmcnc
This test measures the level of corticotropin (ACTH) in your blood 2.5 hours after receiving a glucagon injection to evaluate how well your pituitary and adrenal glands are functioning.
{"Measures ACTH hormone levels in blood after glucagon stimulation","Helps diagnose pituitary and adrenal gland disorders","Requires blood samples taken before and 2.5 hours after glucagon injection","Used to evaluate conditions like Cushing's syndrome and adrenal insufficiency","The test assesses your body's stress response system"}
Key facts at a glance
- LOINC
- 58563-8
- Short code
- ACTH 2.5h Post Glucagon
- Synonyms
- ACTH 2.5h p Gc Plas-mCnc
- Category
- chemistry
- Sample
- plasma
- Container
- Lavender top (EDTA) tube or pink top (EDTA) tube
- Units
- N/A
- Ref low
- 10
- Ref high
- 60
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–120 years
- Method
- Chemiluminescent Immunoassay (CLIA)
- CPT
- 80426
- SNOMED
- 31301003
- Specimen volume
- 1-2 mL of plasma
- Stability
- Plasma should be separated from cells within 1 hour of collection. Store at 2-8°C if analyzing within 24 hours; if delay >24 hours, freeze at -20°C or below. Avoid repeated freeze-thaw cycles
- Hemolysis max
- 50
- Lipemia max
- 300
- Icterus max
- 20
- TAT
- 24–72 hours
- Order
- routine
What is this test?
The ACTH stimulation test with glucagon measures the level of adrenocorticotropic hormone (ACTH), also called corticotropin, in your blood. ACTH is a hormone produced by the pituitary gland that tells your adrenal glands to release cortisol, the stress hormone. This test is performed 2.5 hours after receiving an injection of glucagon, a hormone that normally stimulates ACTH release. By measuring how your body responds, doctors can determine if your pituitary and adrenal glands are working properly.
Why is this test performed?
Your doctor may order this test if you have symptoms of a hormone disorder, such as unexplained fatigue, weight changes, high blood pressure, or excessive hair growth. It helps diagnose conditions including Cushing's syndrome (too much cortisol), Addison's disease (adrenal insufficiency), and pituitary tumors. The glucagon stimulation test is particularly useful for patients who cannot undergo the more common ACTH stimulation test with cosyntropin.
How to prepare for the test
Follow these instructions before your test: Fast (do not eat or drink) for at least 8-12 hours before the test, unless your doctor instructs otherwise. Inform your doctor about all medications you are taking, as certain drugs like steroids, antidepressants, and hormonal treatments may affect results. Tell your doctor if you are pregnant or have diabetes, as these conditions may require special consideration. Avoid strenuous exercise and stress before the test, as these can affect hormone levels. Wear comfortable clothing with sleeves that can be easily rolled up for blood draws.
What happens during the test
The test typically takes 2-3 hours. First, a healthcare professional will draw your initial blood sample (baseline). Then, you will receive a glucagon injection, usually into a muscle or under the skin. You will wait in the clinic for 2.5 hours. At the 2.5-hour mark, another blood sample will be drawn. You may experience mild symptoms from the glucagon, such as nausea, dizziness, or flushing, which are usually temporary. After the second blood draw, you can eat and resume normal activities.
Understanding your results
Your results will show ACTH levels in the baseline sample and the 2.5-hour sample. Your doctor will interpret these results based on your symptoms and medical history. Abnormal results may indicate problems with your pituitary gland, adrenal glands, or hypothalamus. Further testing may be needed to confirm a diagnosis. Do not worry if your results are abnormal—your doctor will explain what they mean and discuss the next steps with you.
Reference range
Reference range varies by laboratory and assay method; typically 10-60 pg/mL (2.2-13.2 pmol/L) for baseline, with adequate response to glucagon stimulation showing increase from baselineN/A
Patient should fast overnight (8-12 hours). Glucagon stimulation test requires specific protocol: administer glucagon 1-2 mg subcutaneously or intramuscularly, collect blood sample 2.5 hours post-administration. Avoid stress, exercise, and smoking before test. Inform about current medications as certain drugs may affect results (glucocorticoids, metyrapone, insulin, oral contraceptives)
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Frequently asked questions
Is the glucagon injection safe?
How long does it take to get results?
Can I take my regular medications before the test?
What should I do if I feel unwell during the test?
Are there any risks with this test?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 14, 2026. · Read our editorial policy