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Alpha-Fetoprotein-L3, Serum/Plasma — Immunoassay, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the AFP-L3 form of alpha-fetoprotein in blood. It may be used as an additional marker in evaluating liver disease or hepatocellular carcinoma risk.

Quick summary

["AFP-L3 is a specialized blood marker that can provide additional information when interpreted with other liver tests and imaging."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
99077-0
Short code
AFP L3 SerPl IA-mCnc
Synonyms
AFP L3 SerPl IA-mCnc
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
10
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82105
SNOMED
394971007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

Alpha-fetoprotein-L3, or AFP-L3, is a form of alpha-fetoprotein, a protein produced mainly by the liver in developing fetuses. This test measures AFP-L3 in serum or plasma. It is different from a standard total alpha-fetoprotein test.

Why might I need this test?

AFP-L3 may be ordered as an additional test when a clinician is evaluating a liver mass, persistent liver abnormalities, or the risk of hepatocellular carcinoma. It is generally interpreted together with imaging, liver function tests, and other clinical findings rather than used alone.

How is the test performed?

A blood sample is drawn and analyzed in the laboratory using an immunoassay. The result reports the amount of AFP-L3 present in the blood.

How do I prepare for the test?

This test usually requires no special preparation. Follow any instructions from your clinician or laboratory, and tell them if you are pregnant because alpha-fetoprotein levels can naturally change during pregnancy.

Reference range

Normal: <10 ng/mLN/A

Specimen: serum

No special preparation required. For PSA, avoid sexual activity for 24-48 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

AFP-L3

Frequently asked questions

Is AFP-L3 the same as a total AFP test?
No. Total AFP measures all circulating alpha-fetoprotein, while this test specifically measures the AFP-L3 form. A total AFP result may sometimes be ordered alongside it.
Does an abnormal AFP-L3 result mean I have liver cancer?
No. Elevated levels can occur in several conditions, including other forms of liver disease. Imaging and additional tests are often needed to determine the cause.
Do I need to fast before this test?
Fasting is usually not required, but preparation instructions can vary by laboratory. Confirm the requirements with the ordering clinician or testing laboratory.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy