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Alpha-Fetoprotein (AFP), Maternal Serum/Plasma — Multiple Gestation Adjusted

Published: August 9, 2026Updated: August 16, 2026

A prenatal blood screening test that measures AFP in maternal serum or plasma and adjusts the result for a multiple-gestation pregnancy.

Quick summary

["This test helps screen for certain fetal conditions, but an abnormal result usually requires further evaluation and does not confirm a diagnosis."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50610-5
Short code
AFP mult gest adj MoM SerPl
Synonyms
AFP mult gest adj MoM SerPl
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
10
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82105
SNOMED
394971007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

Maternal serum alpha-fetoprotein (AFP) is a screening test performed during pregnancy. It measures AFP, a substance made by the developing fetus, and the result is interpreted according to gestational age and adjusted when the pregnancy involves more than one fetus.

Why might I need this test?

This test may be used to screen for fetal open neural tube defects and certain abdominal wall defects. It can also provide information about the risk of some chromosome conditions, although it is not a diagnostic test for these conditions.

How is the test performed?

A blood sample is collected, and AFP is measured in the serum or plasma. The laboratory compares the result with pregnancy-specific ranges and may adjust the interpretation for a multiple-gestation pregnancy.

How do I prepare for the test?

No fasting is usually required, but you should follow the instructions provided by your prenatal care team. Accurate interpretation depends on the gestational age and whether the pregnancy involves one or more fetuses.

Reference range

Normal: <10 ng/mLN/A

Specimen: serum

No special preparation required. For PSA, avoid sexual activity for 24-48 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Maternal serum AFP

Frequently asked questions

Does this test diagnose a fetal condition?
No. It is a screening test, and an abnormal result means that additional evaluation may be needed.
Why is my result adjusted for a multiple-gestation pregnancy?
AFP levels can naturally be higher in pregnancies with more than one fetus. The adjustment helps the laboratory interpret the result more accurately.
Will I need another test if my AFP result is abnormal?
Often, yes. Your prenatal care provider may recommend ultrasound, detailed fetal imaging, genetic counseling, or other diagnostic testing based on the complete result.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy