Skip to content

Free Alpha Subunit, Serum/Plasma — Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the concentration of the free alpha-subunit portion of a protein hormone in serum or plasma.

Quick summary

["This test measures a free hormone component and is best interpreted with the related hormone results and your clinician's assessment."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
100835-8
Short code
Alpha subunit free sp6 p chal SerPl-aCnc
Synonyms
Alpha subunit free sp6 p chal SerPl-aCnc
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
44
Ref high
147
Critical range
— – 100 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84075
SNOMED
27550007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

A free alpha subunit is the unbound portion of the alpha component of certain protein hormones. This test measures its concentration in a blood sample after the laboratory separates serum or plasma from the blood.

Why might I need this test?

Free alpha-subunit measurements may be ordered to help evaluate hormone production or activity, track changes over time, or supplement results for the complete hormone. The reason for testing depends on the specific hormone identified on your laboratory report.

How is the test performed?

A blood sample is drawn from a vein and processed into serum or plasma. The laboratory then measures the free alpha-subunit concentration using a validated assay.

How do I prepare for the test?

Special preparation is often not required, but follow the instructions provided by the laboratory. If the test is part of a challenge or timed collection, the exact timing and any preceding dose or procedure are important.

Reference range

Adult: 44-147 U/LN/A

Specimen: serum

No special preparation required. Avoid fatty meals before the test if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

An abnormal free alpha-subunit level may reflect changes in the production, release, or clearance of the hormone from which it comes. Results should be interpreted with the complete hormone measurement, symptoms, medical history, and the laboratory's reference interval. This result alone does not establish a diagnosis.

Short name

Free alpha subunit

Frequently asked questions

What does “free” mean in this test?
“Free” means the alpha subunit is not bound to another part of the hormone or to a carrier protein. The test measures this unbound portion separately.
Can this test determine whether I am pregnant?
A free alpha-subunit result may be related to pregnancy testing when it is part of a hormone panel, but it should not be used alone to determine pregnancy. The standard pregnancy hormone result and the wording of the laboratory report should guide interpretation.
Why is my result interpreted with other hormone tests?
Free and complete hormone levels can change independently. Comparing them can help your clinician better understand hormone production and activity without relying on one measurement by itself.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy