Ova and Parasite (O&P) Examination, Stool (3 Specimens)
This test measures amylase levels in blood samples collected after receiving Sincalide, a hormone that stimulates pancreatic function.
{"Measures amylase enzyme activity after pancreatic stimulation","Helps evaluate how well the pancreas is functioning","Involves collecting multiple blood samples after Sincalide injection","Used to diagnose pancreatic disorders","The 3rd specimen is taken at a specific time point post-stimulation"}
Key facts at a glance
- LOINC
- 35344-1
- Short code
- Amylase sp3 p sincalide Fld-cCnc
- Synonyms
- Amylase sp3 p sincalide Fld-cCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Critical range
- — – 500 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
This test checks a sample of your stool (bowel movement) for intestinal parasites or their eggs, such as Giardia or Cryptosporidium. Your doctor may order it if you have ongoing diarrhea, stomach cramps, or other digestive problems, especially after traveling or drinking untreated water.
The Amylase test after Sincalide stimulation measures the level of amylase enzyme in your blood. Amylase is produced by the pancreas and helps digest carbohydrates. Sincalide is a synthetic hormone similar to cholecystokinin (CCK), which naturally stimulates the pancreas to release digestive enzymes. By measuring amylase levels after Sincalide administration, doctors can assess how well your pancreas is working.
You will collect small stool samples at home using a special container or kit from your doctor or the laboratory. You typically provide three separate samples on three different days, following the collection instructions carefully, and then return them to the lab to be examined under a microscope.
During this test, you will first receive an injection of Sincalide. Then, your healthcare provider will collect blood samples at specific intervals—typically 30, 60, and 90 minutes after the injection. The '3rd specimen' refers to the third blood sample collected, usually around 90 minutes after Sincalide administration. Each sample is sent to a laboratory to measure amylase activity.
Parasites and their eggs are not passed in every bowel movement, so a single sample can easily miss an infection. Collecting a third specimen on a different day greatly increases the chance of detecting a parasite if one is present, helping your doctor make an accurate diagnosis and choose the right treatment.
The timing of blood collection is crucial because pancreatic response to stimulation follows a specific pattern. The 3rd specimen helps doctors see the later phase of pancreatic enzyme release, providing a more complete picture of pancreatic function. This sequential sampling helps identify if your pancreas responds normally to hormonal stimulation.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is primarily used to evaluate pancreatic exocrine function. Abnormal results may indicate conditions such as chronic pancreatitis, pancreatic insufficiency, or other pancreatic disorders. A normal response shows increasing amylase levels after Sincalide stimulation, while a blunted or absent response may suggest pancreatic dysfunction. Your doctor will interpret these results alongside your clinical symptoms and other diagnostic tests.
Frequently asked questions
Do I need to fast before this test?
Are there any side effects from Sincalide?
How long does the entire test take?
When will I get my results?
Sources
- 1
- 2
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy