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Ova and Parasite (O&P) Examination, Stool (3 Specimens)

Published: August 9, 2026Updated: August 16, 2026

This test measures amylase levels in blood samples collected after receiving Sincalide, a hormone that stimulates pancreatic function.

Quick summary

{"Measures amylase enzyme activity after pancreatic stimulation","Helps evaluate how well the pancreas is functioning","Involves collecting multiple blood samples after Sincalide injection","Used to diagnose pancreatic disorders","The 3rd specimen is taken at a specific time point post-stimulation"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
35344-1
Short code
Amylase sp3 p sincalide Fld-cCnc
Synonyms
Amylase sp3 p sincalide Fld-cCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

This test checks a sample of your stool (bowel movement) for intestinal parasites or their eggs, such as Giardia or Cryptosporidium. Your doctor may order it if you have ongoing diarrhea, stomach cramps, or other digestive problems, especially after traveling or drinking untreated water.

The Amylase test after Sincalide stimulation measures the level of amylase enzyme in your blood. Amylase is produced by the pancreas and helps digest carbohydrates. Sincalide is a synthetic hormone similar to cholecystokinin (CCK), which naturally stimulates the pancreas to release digestive enzymes. By measuring amylase levels after Sincalide administration, doctors can assess how well your pancreas is working.

You will collect small stool samples at home using a special container or kit from your doctor or the laboratory. You typically provide three separate samples on three different days, following the collection instructions carefully, and then return them to the lab to be examined under a microscope.

During this test, you will first receive an injection of Sincalide. Then, your healthcare provider will collect blood samples at specific intervals—typically 30, 60, and 90 minutes after the injection. The '3rd specimen' refers to the third blood sample collected, usually around 90 minutes after Sincalide administration. Each sample is sent to a laboratory to measure amylase activity.

Parasites and their eggs are not passed in every bowel movement, so a single sample can easily miss an infection. Collecting a third specimen on a different day greatly increases the chance of detecting a parasite if one is present, helping your doctor make an accurate diagnosis and choose the right treatment.

The timing of blood collection is crucial because pancreatic response to stimulation follows a specific pattern. The 3rd specimen helps doctors see the later phase of pancreatic enzyme release, providing a more complete picture of pancreatic function. This sequential sampling helps identify if your pancreas responds normally to hormonal stimulation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is primarily used to evaluate pancreatic exocrine function. Abnormal results may indicate conditions such as chronic pancreatitis, pancreatic insufficiency, or other pancreatic disorders. A normal response shows increasing amylase levels after Sincalide stimulation, while a blunted or absent response may suggest pancreatic dysfunction. Your doctor will interpret these results alongside your clinical symptoms and other diagnostic tests.

Frequently asked questions

Do I need to fast before this test?
Yes, you typically need to fast for 8-12 hours before the test. Your healthcare provider will give you specific instructions. Usually, you should not eat or drink anything except water during the fasting period.
Are there any side effects from Sincalide?
Some patients may experience mild side effects such as nausea, abdominal discomfort, or flushing after receiving Sincalide. These symptoms are usually temporary and resolve quickly. Serious reactions are rare.
How long does the entire test take?
The test typically takes about 2-3 hours, including the injection, waiting periods, and blood collection at each time point. You should plan to stay at the testing facility for the duration.
When will I get my results?
Results are usually available within a few days, as each specimen requires laboratory analysis. Your healthcare provider will contact you with the results and explain what they mean for your health.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy