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Antinuclear Antibody (ANA) Test, Blood

Published: August 9, 2026Updated: August 17, 2026

A blood test that detects antinuclear antibodies, which can help evaluate suspected autoimmune conditions such as lupus.

Quick summary

["A positive ANA result may support an autoimmune condition but must be interpreted with your symptoms and other tests."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
104114-4
Short code
A-NAG DBS-cCnc
Synonyms
A-NAG DBS-cCnc
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
2
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
86038
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

The antinuclear antibody (ANA) test looks for antibodies that react with structures inside the body's cells. It is mainly used as part of an evaluation for autoimmune diseases.

Why might I need this test?

Your clinician may order an ANA test if you have symptoms suggesting an autoimmune condition, such as joint pain, unexplained rashes, fatigue, fever, or organ-related symptoms. The result is usually interpreted together with your medical history and other laboratory findings.

How is the test performed?

A blood sample is collected and examined in the laboratory using an antibody-detection method. Depending on the laboratory method, the report may include a positive or negative result and, when appropriate, a titer or staining pattern.

How do I prepare for the test?

No special preparation is usually required. Tell your clinician about any autoimmune disease, recent infection, pregnancy, or medicines that may affect the result, because these factors can influence interpretation.

Reference range

NCBI: SCC <2.0 ng/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ANA test

Frequently asked questions

Does a positive ANA result mean I have lupus?
No. ANA is a screening marker that can be positive in several conditions and in some healthy people. Lupus generally requires additional clinical and laboratory evidence.
Can an ANA result be negative even when an autoimmune disease is present?
Yes. A negative ANA result makes some connective-tissue diseases less likely, but it does not rule out every autoimmune condition. Other targeted antibody tests may be needed.
What should I do with my ANA result?
Share the report with the clinician who ordered it. The result is most useful when reviewed alongside your symptoms, medical history, physical examination, and other laboratory tests.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy