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What is this test?

The centrosomal nuclear IgG pattern test, also known as the anti-centromere antibody test, is a blood test that uses a technique called indirect immunofluorescence to detect specific antibodies in your blood. These antibodies (called IgG anti-centromere antibodies) target the centromere region of chromosomes, which is responsible for proper chromosome separation during cell division.

How is the test performed?

A healthcare professional will draw a small blood sample from a vein in your arm. The sample is then sent to a laboratory where technicians will mix it with special cells that have visible centromeres. If anti-centromere antibodies are present in your blood, they will bind to these cells. A fluorescent dye is added, and the mixture is examined under a microscope. The highest dilution (titer) at which antibodies can still be detected is reported as the result.

What do the results mean?

Results are typically reported as a titer (for example, 1:40, 1:160). A positive result (presence of antibodies at significant titers) usually indicates an autoimmune disorder, most commonly CREST syndrome (a limited form of systemic sclerosis). CREST stands for Calcinosis, Raynaud's phenomenon, Esophageal dysmotility, Sclerodactyly, and Telangiectasia. In some cases, positive results may also be associated with other conditions like primary biliary cholangitis or other autoimmune diseases.

Is fasting required?

No, fasting is not typically required for this test. You can eat and drink normally before the blood draw. However, always follow any specific instructions provided by your healthcare provider.