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Anasmoothnuclenvtitrfld

Published: August 9, 2026Updated: August 16, 2026

This blood test detects antibodies targeting the smooth nuclear envelope, helping diagnose autoimmune conditions affecting the liver and connective tissues.

Quick summary

{"The test identifies a specific ANA pattern called smooth nuclear envelope or rim pattern","Positive results are commonly associated with primary biliary cholangitis and lupus","The test uses a blood sample and does not require fasting","Results are reported as a titer (ratio) indicating antibody concentration","Follow-up testing may be needed to confirm a diagnosis"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
42213-9
Short code
ANA smooth nucl env Titr Fld
Synonyms
ANA smooth nucl env Titr Fld
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
86038
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

The Smooth Nuclear Envelope Antibody test is a specialized blood test that detects antinuclear antibodies (ANA) targeting the nuclear envelope—the membrane surrounding the cell nucleus. This test uses indirect immunofluorescence to identify a specific pattern called the 'smooth nuclear envelope' or 'rim pattern,' where antibodies create a smooth, ring-like staining around the nucleus.

How is the test performed?

A healthcare provider will draw a blood sample from a vein in your arm. The sample is then sent to a laboratory where technicians will mix it with special cells to detect the presence and strength (titer) of smooth nuclear envelope antibodies. The test does not require fasting or special preparation.

What do the results mean?

Results are typically reported as a titer (such as 1:40, 1:80, 1:160, etc.) with a corresponding pattern. A higher titer generally indicates a stronger immune response. The smooth nuclear envelope pattern, when present at significant titers, suggests your immune system may be attacking your own cells, which is characteristic of certain autoimmune diseases.

Are there any risks?

The risks are minimal and similar to routine blood tests. You may experience slight bruising, soreness, or bleeding at the puncture site. Some people may feel lightheaded during or after the blood draw. These side effects usually resolve quickly.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Do I need to fast before this test?
No, fasting is not required for this test. You can eat and drink normally before having your blood drawn.
Is a positive result always a sign of disease?
Not necessarily. Low titers can sometimes be found in healthy individuals, especially older adults. Your healthcare provider will interpret your results in the context of your symptoms, medical history, and other test findings.
How long does it take to get results?
Results are typically available within 3-7 days, depending on the laboratory. Your healthcare provider's office will contact you with the results.
What happens if my test is positive?
A positive result means antibodies were detected. Your healthcare provider will likely order additional tests to determine the cause, such as liver function tests, other antibody tests, or imaging studies. They will discuss the next steps based on your complete clinical picture.
Can this test diagnose a specific condition?
This test alone cannot diagnose a specific disease. It is one piece of information used alongside your symptoms, physical examination, and other laboratory tests to help identify autoimmune conditions, particularly primary biliary cholangitis or lupus.
  1. 1
    American College of Rheumatology - ANA TestingSource
  2. 2
    National Institute of Diabetes and Digestive and Kidney Diseases - Primary Biliary CholangitisSource
  3. 3
    Mayo Clinic - Antinuclear Antibody TestSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy