Skip to content

Androstsp7pchalserplmcnc

Published: August 9, 2026Updated: August 15, 2026

This test measures the level of androstenedione, a hormone that serves as a building block for testosterone and estrogen, in your blood after a stimulation challenge.

Quick summary

{"Androstenedione is a hormone produced by the adrenal glands and gonads","This test helps diagnose conditions like PCOS, adrenal disorders, and hormone imbalances","The test involves collecting blood samples after a hormonal stimulation challenge","Results help doctors evaluate causes of abnormal hair growth, menstrual irregularities, or fertility issues","No special preparation is typically needed beyond following your doctor's instructions for the challenge test"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
16383-2
Short code
ANDRO7
Synonyms
Androst sp7 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top serum tube or serum separator tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–99 years
Method
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) or Immunoassay
CPT
82157
SNOMED
250586003
Specimen volume
1.0 mL serum
Stability
Serum stable for 24 hours at room temperature (20-25°C), 7 days refrigerated at 2-8°C, and up to 6 months frozen at -20°C or below. Avoid repeated freeze-thaw cycles.
Hemolysis max
100
Lipemia max
100
Icterus max
50
TAT
24–72 hours
Order
routine

What is this test?

The androstenedione test measures the amount of androstenedione (also called A4) in your blood serum or plasma. Androstenedione is a steroid hormone produced primarily by the adrenal glands in both men and women, and also by the ovaries in women and testes in men. It serves as a precursor (building block) for other important hormones like testosterone and estrogen.

What does '7th specimen post XXX challenge' mean?

This indicates that the test is performed after a stimulation challenge test (such as an ACTH stimulation test). Multiple blood samples are collected over time to track how your body responds to the challenge. The '7th specimen' refers to the seventh blood sample taken during this dynamic hormone testing process, which helps doctors understand how well your adrenal glands and other hormone-producing organs are functioning.

Why might I need this test?

Your doctor may order this test if you have symptoms of hormone imbalance, such as: irregular menstrual periods, excessive hair growth (hirsutism), acne, difficulty becoming pregnant, or signs of puberty problems. It helps evaluate conditions like polycystic ovary syndrome (PCOS), congenital adrenal hyperplasia, adrenal tumors, or other disorders affecting hormone production.

How is the test performed?

A healthcare professional will collect a blood sample from a vein in your arm using a small needle. The sample will be sent to a laboratory for analysis. If this is part of a challenge test, you may receive a medication (like ACTH) to stimulate hormone production, and multiple samples will be taken at specific time intervals.

Reference range

Reference ranges for androstenedione vary by age, sex, and assay method. Post-challenge 7th specimen values should be interpreted in the context of the specific stimulation or suppression protocol used (e.g., ACTH stimulation, dexamethasone suppression, GnRH challenge). Baseline and stimulated values are interpreted by the ordering physician.N/A

Specimen: serum

No fasting required. Specimen should be collected as the 7th timed sample following the specified challenge protocol. Patient should remain at rest prior to collection. Stress and physical exertion can affect adrenal steroid production and should be minimized prior to specimen collection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Do I need to fast before this test?
Follow your doctor's specific instructions. Some hormone tests require fasting, while others do not. Your healthcare provider will inform you about any necessary preparation before the test.
Are there any risks with this blood test?
The risks are minimal and include slight pain, bruising, or bleeding at the needle site. Some people may feel dizzy or lightheaded during or after the blood draw.
How long does it take to get results?
Results typically take 1-3 days, depending on the laboratory. Your doctor will contact you with the results or discuss them at your follow-up appointment.
What do abnormal results mean?
Abnormal results do not always indicate a serious condition. Your doctor will interpret your results based on your symptoms, medical history, and other test results. Additional testing may be needed to confirm a diagnosis.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy