16S rRNA Gene Sequencing from Bacterial DNA (for Microbiome Analysis)
This test analyzes genetic material from bacteria in your sample to identify specific bacterial species present. It helps assess microbial communities, often used in research or specialized clinical settings.
["This test identifies bacteria in your sample by examining their unique genetic material."]
Key facts at a glance
- LOINC
- 76575-0
- Short code
- 16SrRNA
- Synonyms
- Bact 16S rRNA Spec Ql NAA+probe
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, DNA-free, leak-proof container (e.g., cryovial or sterile tube without preservatives)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 0 years
- Method
- probe-amp-tar
- CPT
- 87801
- SNOMED
- 122450005
- Specimen volume
- 1-2 mL of serum or appropriate specimen volume per laboratory protocol
- Stability
- Room temperature: 2 hours; Refrigerated (2-8°C): 24 hours; Frozen (-70°C or below): 30 days for nucleic acid preservation
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 24–72 hours
- Order
- routine
What This Test Measures
The test focuses on the 16S rRNA gene, a conserved region in bacterial DNA that varies slightly among different species. By sequencing this gene, healthcare providers can determine which bacteria are present in your sample, even if they are not cultured or visible under a microscope.
How the Sample Is Collected
Your sample (likely a stool, saliva, or other bodily fluid) is sent to a lab where bacteria are extracted from it. The lab then isolates the 16S rRNA gene and sequences it to create a profile of the bacterial species present.
When This Test Might Be Ordered
This test is typically used in advanced diagnostic or research settings, such as microbiome studies, infectious disease investigations, or when traditional culture methods are insufficient to identify bacteria.
What to Expect Before the Test
No special preparation is usually required, but you may need to follow instructions specific to your sample type (e.g., fasting for stool samples). Avoid certain medications or dietary restrictions if directed by your healthcare provider.
Reference range
Negative (No bacterial 16S rRNA detected)N/A
No special patient preparation required. Specimen should be collected prior to initiation of antimicrobial therapy when possible to maximize diagnostic yield.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Bacterial 16S rRNA Sequencing
Frequently asked questions
This test provides detailed insights into your microbial environment, which can be critical for diagnosing infections, evaluating gut health, or guiding treatments for conditions like inflammatory bowel disease or antibiotic-associated diarrhea.
How long will it take to get results?
Is this test covered by insurance?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy