16S rRNA Gene Detection in Bacterial DNA (via Probe-Based Assay)
This test checks for specific bacteria in your body by analyzing a small genetic segment called 16S rRNA, commonly used to identify unknown microbes.
["Helps diagnose infections by identifying bacteria present in your specimen."]
Key facts at a glance
- LOINC
- 76590-9
- Short code
- 16SRNA
- Synonyms
- Bact 16S rRNA XXX.tiss NAA+probe
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, DNase/RNase-free cryogenic vial or sterile screw-cap container
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- probe-amp-tar
- CPT
- 87801
- SNOMED
- 442538003
- Specimen volume
- Tissue biopsy sample of approximately 0.5-1 cm³ or 50-100 mg minimum
- Stability
- Store at 2-8°C for up to 24 hours; for prolonged storage, freeze at -70°C or below. Avoid repeated freeze-thaw cycles.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 24–72 hours
- Order
- routine
What This Test Measures
The test uses a specialized probe designed to target the 16S ribosomal RNA gene—a conserved region found in nearly all bacteria. When bacteria are present in your sample, the probe binds to their genetic material, allowing for detection and identification.
How the Test Works
Your sample (likely collected from a bodily fluid or tissue) is processed to extract bacterial DNA. The probe detects the presence of bacterial 16S rRNA sequences, which can help differentiate between various bacterial species.
Common Uses
This test is often used in microbiology labs to identify unknown bacteria in clinical samples, such as blood, sputum, or other bodily fluids, aiding in guiding appropriate antibiotic treatment.
Sample Collection and Preparation
Your healthcare provider will collect a sample (e.g., blood, urine, or swab) based on your clinical symptoms or suspected infection. The sample is then sent to a lab where bacterial DNA is extracted and tested for the presence of 16S rRNA sequences.
Reference range
Negative (no bacterial 16S rRNA detected). Quantitative results reported as [#/mass] of bacterial target detected per mass of tissue.N/A
No fasting required. Specimen collection site should be properly decontaminated to avoid contamination with environmental flora. Tissue biopsy should be collected under aseptic conditions.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Bacterial 16S rRNA Probe Test
Frequently asked questions
Why might my doctor order this test?
What does a positive result mean?
How long will results take?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy