Complete Blood Count (CBC): What You Need to Know About This Common Blood Test
This test identifies anaerobic (oxygen-hating) bacteria in bile fluid to help diagnose infections of the gallbladder and bile ducts.
{"The test detects anaerobic bacteria that may cause gallbladder or bile duct infections","Bile fluid is collected during a medical procedure such as ERCP or gallbladder drainage","Results help doctors choose the right antibiotics to treat the infection","Anaerobic bacteria grow in low-oxygen environments and often require special culture methods","A negative result does not completely rule out infection"}
Key facts at a glance
- LOINC
- 87934-6
- Short code
- BILEANA
- Synonyms
- Bacteria Bifl Anaerobe Cult
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, leak-proof, anaerobic transport container or sterile screw-capped tube; specimen should be transported in anaerobic transport medium to preserve viability of oxygen-sensitive organisms
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- anaerobic-culture
- CPT
- 87075
- SNOMED
- 44162008
- Specimen volume
- 1-5 mL of bile fluid; minimum 1 mL recommended for adequate anaerobic culture
- Stability
- Specimen should be processed immediately upon receipt. If delayed, refrigerate at 2-8°C for no more than 2 hours. Anaerobic organisms are highly sensitive to oxygen exposure and delays in processing may result in loss of viability
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 72–168 hours
- Order
- routine
The complete blood count (CBC) is a common blood test that measures the different cells in your blood, including red blood cells, white blood cells, and platelets. It gives your doctor a broad picture of your overall health and can help detect a wide range of conditions, such as anemia, infections, and blood disorders.
The Anaerobic Bacteria Culture from Bile Fluid test looks for specific bacteria that thrive in environments without oxygen (anaerobes) in your bile fluid. Bile is a digestive fluid produced by the liver and stored in the gallbladder. When bacteria infect the bile system, it can cause conditions like cholecystitis (gallbladder inflammation) or cholangitis (bile duct infection).
A healthcare professional will draw a small sample of blood, usually from a vein in your arm, using a thin needle. The collection typically takes only a few minutes, and the sample is then sent to a laboratory for analysis. For infants and young children, the blood is sometimes taken from the heel instead.
Bile fluid is not collected from blood (serum). Instead, it is obtained through a medical procedure, most commonly during Endoscopic Retrograde Cholangiopancreatography (ERCP), a procedure that examines the bile ducts, or through a needle inserted into the gallbladder (percutaneous cholecystostomy). The collected fluid is then placed in special containers that prevent oxygen exposure, as anaerobic bacteria die when exposed to air.
This test is important because it provides key information about the cells that make up your blood. It can help diagnose anemia, identify infections, screen for certain blood disorders, monitor ongoing medical conditions and treatments, and serve as part of a routine checkup to catch health problems early.
Anaerobic infections in the bile system can be serious and require specific antibiotic treatment. Regular antibiotics may not work against these bacteria. This test helps your doctor identify exactly which bacteria are causing the infection so they can prescribe the most effective treatment. Common anaerobic bacteria found in bile infections include Bacteroides, Clostridium, and Fusobacterium species.
During the test, you will be seated while a technician wraps a band around your upper arm, cleans the puncture site with an antiseptic, and inserts a small needle to collect blood into a tube. You may feel a brief sting or pinch. Afterward, pressure is applied to the site and a bandage is placed. Most people can return to their normal activities right away.
The laboratory will place your bile fluid sample in special culture bottles that create an oxygen-free environment. The sample is incubated for several days, as anaerobic bacteria grow slowly. If bacteria grow, further tests are done to identify the exact type of bacteria and determine which antibiotics will be most effective (this is called antibiotic susceptibility testing).
Reference range
No anaerobic bacteria isolated. Results reported as 'No growth of anaerobic bacteria' or with identification of specific organisms isolated (e.g., Bacteroides fragilis, Clostridium perfringens, Peptostreptococcus spp.)N/A
No special patient preparation is typically required. Bile fluid is usually obtained via endoscopic retrograde cholangiopancreatography (ERCP) or percutaneous transhepatic cholangiography (PTC). Inform the physician of any recent antibiotic use as it may affect culture results
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
A positive culture result confirms there is a bacterial infection in the bile system and identifies the specific anaerobic bacteria causing it. This information is critical for selecting targeted antibiotic therapy. Common conditions associated with anaerobic bacteria in bile include acute cholecystitis, ascending cholangitis, and biliary stent infections. A negative result may indicate that the infection is caused by other organisms, is viral, or that antibiotics have already started working.
Frequently asked questions
Is the test painful?
How long does it take to get results?
Does a negative result mean I don't have an infection?
What happens after the bacteria are identified?
Sources
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy