Understanding Your Laboratory Test: Why It Is Done, How It Works, and What Your Results Mean
This test checks your blood for harmful bacteria that can grow without oxygen (anaerobes).
{"A blood sample is taken and sent to a lab to grow bacteria","It helps find infections caused by anaerobes that don't need oxygen","Results help doctors choose the right antibiotic treatment","The test takes several days to complete","It is used when a bacterial bloodstream infection is suspected"}
Key facts at a glance
- LOINC
- 45276-3
- Short code
- ANBC25
- Synonyms
- Bacteria Bld Anaerobe Cult 25 Deg
- Category
- chemistry
- Sample
- whole-blood
- Container
- Anaerobic blood culture bottle (e.g., BacT/ALERT FN Plus, BACTEC Anaerobic/F, VersaTREK Redox 2 Anaerobic); sterile collection syringe or butterfly needle set
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 0 years
- Method
- anaerobic-culture-deg-c-incubation
- CPT
- 87040
- SNOMED
- 122450003
- Specimen volume
- Adults: 8-10 mL per anaerobic bottle (20-30 mL total per set with aerobic bottle); Pediatric: 1-4 mL per bottle based on patient weight; Neonates: 0.5-1 mL per bottle
- Stability
- Inoculated bottles must be transported to the laboratory within 2 hours at room temperature (20-25°C). Do not refrigerate or incubate at 25°C for prolonged periods before transport. Once in the laboratory, bottles are incubated continuously in the monitored blood culture instrument for up to 5-7 days.
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 48–168 hours
- Order
- routine
This is a laboratory test that measures specific substances in a sample of your blood, urine, or other body fluid. It helps your doctor check how your body is working, screen for certain health conditions, or monitor how well an ongoing treatment is going.
A blood anaerobe culture is a laboratory test where your blood sample is placed in special containers that encourage anaerobic bacteria (bacteria that can live without oxygen) to grow. The sample is incubated at 25°C to help identify specific types of anaerobic bacteria that may be causing an infection in your bloodstream.
Your doctor may order this test if you have symptoms that need a closer look, if you have risk factors for a certain condition, before starting a new medication, or as part of a routine checkup to make sure everything in your body is working as expected.
Your doctor may order this test if you have symptoms of a bloodstream infection, such as fever, chills, rapid heartbeat, or confusion. It helps identify if anaerobic bacteria are causing your infection, which is important because different bacteria require different treatments.
Most often, the test is done with a small sample collected in a clinic or laboratory. A trained staff member draws blood from a vein in your arm or collects a urine sample, labels it, and sends it to the lab for analysis. The procedure itself usually takes only a few minutes.
A healthcare professional will draw blood from a vein in your arm, usually from multiple bottles to increase the chance of finding bacteria. The blood is then sent to a laboratory where it is placed in special culture bottles and incubated. Laboratory technicians monitor the sample daily to see if bacteria grow, and if they do, they identify the specific type.
Your results show whether the measured values fall inside or outside the normal range. Readings that are higher or lower than expected may point to a health condition, but a single test rarely tells the whole story. Your doctor will explain what your numbers mean together with your symptoms, medical history, and other test results.
A positive result means anaerobic bacteria were found in your blood, indicating a bacterial infection. A negative result means no anaerobic bacteria were detected, but it does not completely rule out infection—it may take time for bacteria to grow, or another type of organism may be involved. Your doctor will interpret the results together with your symptoms and other test findings.
Reference range
No anaerobic bacteria isolated; no growth observed. Identification reported qualitatively as organism name when present (e.g., Bacteroides fragilis, Clostridium perfringens, Fusobacterium nucleatum, Peptostreptococcus spp., Prevotella spp.)N/A
No fasting required. Skin antisepsis at the venipuncture site is critical (use 2% chlorhexidine or 70% isopropyl alcohol). Disinfect blood culture bottle tops prior to inoculation. Collect prior to initiating antimicrobial therapy when possible. Obtain multiple sets from separate venipuncture sites (typically 2-3 sets) to differentiate true bacteremia from contamination.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is crucial for diagnosing bacteremia (bacteria in the blood) caused by anaerobic organisms. Anaerobic infections can arise from various sources, including wounds, dental infections, gastrointestinal perforations, or abscesses. Identifying the specific anaerobic bacterium helps guide targeted antibiotic therapy, as some anaerobes are resistant to certain antibiotics. This test is particularly important for patients with severe infections, sepsis, or those who have not responded to standard antibiotic treatments.
Frequently asked questions
How long does it take to get results?
Does this test hurt?
Do I need to fast before this test?
What happens if anaerobic bacteria are found?
Sources
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy