Understanding Your Laboratory Test: What It Measures, How It Works, and Why Your Doctor Ordered It
This test identifies aerobic bacteria found in a bone sample to help diagnose bone infections.
{"The test detects bacteria in bone tissue to diagnose infections","Results help doctors choose the right antibiotic treatment","A bone sample is needed for this test, not blood"}
Key facts at a glance
- LOINC
- 17877-2
- Short code
- BCB6
- Synonyms
- Bacteria Bone Aerobe Cult org #6
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, leak-proof container without preservative; aerobic and anaerobic culture transport system; sterile screw-cap tube or cup
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- aerobic-culture
- CPT
- 87077
- SNOMED
- 416675000
- Specimen volume
- Minimum 1-2 mL of bone tissue or aspirated fluid; multiple specimens from different sites preferred for orthopedic infections
- Stability
- Room temperature: 2 hours; refrigerated (2-8°C): 24 hours. Do not freeze. Transport to laboratory as soon as possible for optimal organism viability.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–168 hours
- Order
- routine
What is this test, and what does it measure? This laboratory test examines a sample from your body so your healthcare provider can screen for disease, evaluate how well your organs are working, and make informed decisions about your ongoing care.
The Bone Bacteria Culture test (also called aerobic bone culture) is a laboratory test that identifies specific types of bacteria growing in a sample of bone tissue. This test is used to diagnose bone infections, such as osteomyelitis, which is an infection of the bone that can cause pain, swelling, and serious complications if left untreated.
How is the sample collected? A trained professional will gather your sample, most often a small amount of blood drawn from a vein in your arm, though some tests use urine, a throat swab, or another specimen, all following careful steps to keep you comfortable and the sample safe.
A small sample of bone tissue is collected by your doctor through a biopsy procedure. This is usually done under local anesthesia. The bone sample is then sent to a laboratory where it is placed in a special environment that allows aerobic (oxygen-requiring) bacteria to grow. Laboratory technicians monitor the sample over several days to identify any bacteria present.
Why is this test performed? Your doctor orders this test to look for signs of illness, confirm or rule out a specific diagnosis, monitor an existing condition, or track how well a treatment is working so that your care plan can be adjusted when needed.
This test is performed when your doctor suspects a bone infection. Symptoms of bone infection may include persistent bone pain, fever, swelling, redness, warmth in the affected area, and difficulty moving the affected limb. Early and accurate identification of the bacteria helps doctors prescribe the most effective antibiotic treatment.
Reference range
No growth of pathogenic bacteria. Results reported as organism identification with colony morphology and quantification if applicable.N/A
No fasting required. Patient should inform healthcare provider of all current medications, especially antibiotics, as prior antibiotic therapy may affect culture results. Bone specimen collection should be performed under sterile conditions.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is primarily used to diagnose osteomyelitis (bone infection) and determine the specific bacteria causing the infection. The results guide antibiotic selection and treatment duration. Common bacteria that may be identified include Staphylococcus aureus, Streptococcus species, and Gram-negative bacteria. A negative result does not completely rule out infection, as some bacteria are difficult to grow or require special conditions.
Frequently asked questions
How long does it take to get results?
Does a negative result mean I don't have an infection?
What happens after bacteria are identified?
Is the bone biopsy painful?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy