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Understanding Your Laboratory Test: What It Measures, How It Works, and Why Your Doctor Ordered It

Published: August 9, 2026Updated: August 16, 2026

This test identifies aerobic bacteria found in a bone sample to help diagnose bone infections.

Quick summary

{"The test detects bacteria in bone tissue to diagnose infections","Results help doctors choose the right antibiotic treatment","A bone sample is needed for this test, not blood"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17877-2
Short code
BCB6
Synonyms
Bacteria Bone Aerobe Cult org #6
Category
chemistry
Sample
serum
Container
Sterile, leak-proof container without preservative; aerobic and anaerobic culture transport system; sterile screw-cap tube or cup
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
aerobic-culture
CPT
87077
SNOMED
416675000
Specimen volume
Minimum 1-2 mL of bone tissue or aspirated fluid; multiple specimens from different sites preferred for orthopedic infections
Stability
Room temperature: 2 hours; refrigerated (2-8°C): 24 hours. Do not freeze. Transport to laboratory as soon as possible for optimal organism viability.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
48–168 hours
Order
routine

What is this test, and what does it measure? This laboratory test examines a sample from your body so your healthcare provider can screen for disease, evaluate how well your organs are working, and make informed decisions about your ongoing care.

The Bone Bacteria Culture test (also called aerobic bone culture) is a laboratory test that identifies specific types of bacteria growing in a sample of bone tissue. This test is used to diagnose bone infections, such as osteomyelitis, which is an infection of the bone that can cause pain, swelling, and serious complications if left untreated.

How is the sample collected? A trained professional will gather your sample, most often a small amount of blood drawn from a vein in your arm, though some tests use urine, a throat swab, or another specimen, all following careful steps to keep you comfortable and the sample safe.

A small sample of bone tissue is collected by your doctor through a biopsy procedure. This is usually done under local anesthesia. The bone sample is then sent to a laboratory where it is placed in a special environment that allows aerobic (oxygen-requiring) bacteria to grow. Laboratory technicians monitor the sample over several days to identify any bacteria present.

Why is this test performed? Your doctor orders this test to look for signs of illness, confirm or rule out a specific diagnosis, monitor an existing condition, or track how well a treatment is working so that your care plan can be adjusted when needed.

This test is performed when your doctor suspects a bone infection. Symptoms of bone infection may include persistent bone pain, fever, swelling, redness, warmth in the affected area, and difficulty moving the affected limb. Early and accurate identification of the bacteria helps doctors prescribe the most effective antibiotic treatment.

Reference range

No growth of pathogenic bacteria. Results reported as organism identification with colony morphology and quantification if applicable.N/A

Specimen: serum

No fasting required. Patient should inform healthcare provider of all current medications, especially antibiotics, as prior antibiotic therapy may affect culture results. Bone specimen collection should be performed under sterile conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is primarily used to diagnose osteomyelitis (bone infection) and determine the specific bacteria causing the infection. The results guide antibiotic selection and treatment duration. Common bacteria that may be identified include Staphylococcus aureus, Streptococcus species, and Gram-negative bacteria. A negative result does not completely rule out infection, as some bacteria are difficult to grow or require special conditions.

Frequently asked questions

How long does it take to get results?
Bacterial culture results typically take 2-7 days. Some bacteria grow slowly and may require more time for identification. Your doctor will contact you when results are available.
Does a negative result mean I don't have an infection?
Not necessarily. Some bacteria are difficult to grow in laboratory conditions, or the infection may be caused by organisms that require special growth conditions. Your doctor will interpret the results along with your symptoms and other test findings.
What happens after bacteria are identified?
Once the specific bacteria are identified, your doctor may perform additional tests called susceptibility or sensitivity testing. This determines which antibiotics will be most effective against the specific bacteria found in your bone sample.
Is the bone biopsy painful?
The procedure is performed under local anesthesia, so you should not feel pain during the biopsy. You may experience some discomfort or soreness at the biopsy site afterward, which can be managed with pain relievers as recommended by your doctor.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy