Skip to content

Understanding Your Laboratory Test: What It Is, How It Is Performed, and How to Get Ready

Published: August 9, 2026Updated: August 16, 2026

This test identifies and grows aerobic bacteria from a sample collected from your airways to diagnose respiratory infections.

Quick summary

{"A sample from your bronchial tubes is collected and tested for bacteria","Results help doctors choose the right antibiotic treatment","The test takes 2-5 days to complete","No special preparation is needed before sample collection","Results show which specific bacteria are causing your infection"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
604-9
Short code
BACBCULT
Synonyms
Bacteria Bronch Aerobe Cult
Category
chemistry
Sample
serum
Container
Sterile, leak-proof, screw-cap container or sterile transport tube. For bronchoscopy specimens: sterile sputum cup or specific bronchoalveolar lavage (BAL) collection container. Anaerobic transport media if anaerobes are suspected.
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
aerobic-culture
CPT
87071
SNOMED
398252004
Specimen volume
Bronchial washing/aspirate: minimum 1-2 mL; Bronchoalveolar lavage (BAL): 10-50 mL preferred; Bronchial brushings: brush placed in 1 mL sterile saline or transport medium; Transbronchial biopsy: tissue in sterile saline.
Stability
Transport to laboratory within 2 hours at room temperature for optimal organism recovery. If delay anticipated, refrigerate at 2-8°C and process within 24 hours. Specimens should not be frozen. For fastidious organisms (e.g., Neisseria, Haemophilus), immediate processing or use of appropriate transport medium is essential.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–120 hours
Order
routine

A laboratory test is an analysis performed on a sample of your blood, urine, or other body fluid to help your healthcare provider evaluate your overall health, screen for certain conditions, diagnose an illness, or monitor how well a treatment is working over time.

The Bronchial Bacteria Culture test is a laboratory test that looks for harmful bacteria in a sample taken from your bronchial tubes or lungs. Your doctor may order this test if you have symptoms of a respiratory infection such as persistent cough, fever, difficulty breathing, or chest congestion. The sample is collected through a procedure called bronchoscopy or by collecting sputum (mucus you cough up).

Most samples are collected by a trained phlebotomist, who draws a small amount of blood from a vein in your arm using a sterile needle; the process usually takes only a few minutes. Depending on the test ordered, you may instead be asked to provide a urine specimen, a throat or nasal swab, or another sample at the collection site.

There are two common ways to collect a bronchial sample. The first is through bronchoscopy, where a thin tube with a camera is inserted through your nose or mouth into your airways to collect fluid or tissue. The second is by collecting sputum that you cough up from your lungs. Your healthcare provider will determine which method is appropriate based on your condition.

Once your sample arrives at the laboratory, trained technologists examine it using specialized instruments and chemical reagents to measure specific substances, count blood cells, or detect signs of infection or disease. Results are carefully reviewed for accuracy, then sent securely to your healthcare provider, who interprets them together with your medical history and explains what they mean for you.

In the lab, the sample is placed in special containers with nutrients that allow bacteria to grow. The laboratory technicians monitor the sample for 2-5 days to see which types of bacteria appear. If bacteria grow, they are identified and tested to see which antibiotics can effectively treat the infection. This process is called 'culture' and 'sensitivity testing.'

Preparation depends on the type of test your provider ordered. Some tests require fasting for 8 to 12 hours, so you should not eat or drink anything except water before your appointment, while others have no special requirements. Drink plenty of water unless instructed otherwise, wear a sleeve that rolls up easily, and tell the staff about any medications or supplements you take, since some can affect your results.

For sputum collection, rinse your mouth with water before collecting the sample to avoid contamination. Do not eat or drink before providing the sample if you're having a bronchoscopy. Follow all specific instructions given by your healthcare provider. Inform your doctor about any antibiotics you are currently taking, as they may affect the test results.

Reference range

No growth of pathogenic bacteria. Normal upper respiratory flora may be present. Results reported as: No growth, Normal flora, or identification of specific organisms with colony quantification (e.g., rare, few, moderate, many).N/A

Specimen: serum

No special patient preparation required. Patient should rinse mouth with water prior to specimen collection if possible to reduce oral contamination. Antibiotic therapy history should be documented, as prior antimicrobial use may affect culture results. Bronchoscopy should be performed by qualified personnel under appropriate sedation per institutional protocols.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is crucial for diagnosing bacterial pneumonia, bronchitis, and other lower respiratory tract infections. It helps identify the exact bacteria causing your infection, allowing your doctor to prescribe the most effective antibiotic. This targeted approach leads to better treatment outcomes and helps prevent antibiotic resistance. The test is particularly important for people with weakened immune systems, chronic lung diseases, or those who have not responded to initial antibiotic treatment.

Frequently asked questions

Will the test be painful?
If you have a bronchoscopy, you may experience some discomfort during the procedure, but you will receive medication to help you relax and to numb your throat. Scollection is generally painless.
How long does it take to get results?
Preliminary results may be available in 2-3 days, but a complete analysis including bacterial identification and antibiotic sensitivity testing typically takes 5-7 days.
What do the results mean?
A positive result means bacteria were found and identified, which helps guide your treatment. A negative result means no harmful bacteria were detected, but this does not rule out infection entirely—your doctor will interpret the results along with your symptoms and other tests.
Do I need to stop taking antibiotics before the test?
Do not stop taking antibiotics unless your doctor specifically instructs you to do so. Taking antibiotics before the test may affect the results.
  1. 1
  2. 2

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy