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Understanding Your Laboratory Test: What Patients Need to Know

Published: August 9, 2026Updated: August 16, 2026

This laboratory test detects and identifies anaerobic (oxygen-sensitive) bacteria in body fluid samples to help diagnose infections caused by microorganisms that do not require oxygen to grow.

Quick summary

{"Detects anaerobic bacteria that can cause deep tissue infections and abscesses","Helps doctors choose the right antibiotic by identifying the specific bacteria causing infection","Sample collection requires special handling to keep bacteria alive for accurate testing","Results typically take 2-7 days as bacteria need time to grow in the lab"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17957-2
Short code
ANABID2
Synonyms
Bacteria Fld Anaerobe Cult org #2
Category
chemistry
Sample
serum
Container
Sterile, leak-proof, anaerobic transport container (e.g., anaerobic transport vial, sterile screw-cap tube, or Port-A-Cul transport system). Anaerobic transport medium should be used to maintain viability of obligate anaerobes
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
anaerobic-culture
CPT
87076
SNOMED
415125008
Specimen volume
Minimum 1–2 mL of body fluid; larger volumes (3–10 mL) preferred for adequate recovery, especially from normally sterile body sites
Stability
Specimen should be transported to the laboratory immediately (within 15–30 minutes) at room temperature. If processing is delayed, store at room temperature in anaerobic transport medium for up to 24–48 hours. Do not refrigerate, as cold temperatures are detrimental to anaerobic organisms
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
48–120 hours
Order
routine

What is this test, and why has my healthcare provider ordered it for me?

The Anaerobic Bacteria Culture Test is a laboratory test that detects and identifies anaerobic bacteria in body fluid samples. Anaerobic bacteria are microorganisms that can grow without oxygen. Some anaerobic bacteria can cause serious infections in deep tissues, wounds, abscesses, blood, and other body fluids. This test helps doctors find the exact cause of an infection so they can prescribe the most effective treatment.

How will my sample be collected, and what should I expect during the process?

The sample is collected from the affected area by your healthcare provider. This may include fluid from an abscess, wound, blood, cerebrospinal fluid, pleural fluid, or other body fluids. The collection must be done carefully to prevent the sample from being exposed to oxygen, as this can kill anaerobic bacteria and affect test accuracy. The sample is placed in a special container that maintains anaerobic conditions and is sent to the laboratory quickly.

What happens to my sample once it arrives at the laboratory for testing?

In the lab, the sample is placed in special growth environments that support anaerobic bacteria. The lab monitors the sample for several days to see if bacteria grow. When bacteria are found, they are identified through various methods, and sensitivity testing may be performed to determine which antibiotics will be most effective against the specific bacteria.

Reference range

No anaerobic bacteria isolatedN/A

Specimen: serum

No special patient preparation required. If possible, specimens should be collected prior to administration of antibiotics. For body fluid collection, standard sterile technique (e.g., sterile needle aspiration of synovial, pleural, peritoneal, pericardial, or CSF fluid) must be followed

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is important for diagnosing infections caused by anaerobic bacteria, which often occur in deep tissues, surgical wounds, abscesses, and areas with poor blood supply. Common anaerobic infections include bacterial vaginosis, intra-abdominal infections, lung abscesses, and certain types of blood infections. Identifying the specific anaerobic bacteria helps doctors prescribe targeted antibiotic therapy rather than using broad-spectrum antibiotics, which reduces the risk of antibiotic resistance and improves treatment outcomes.

Frequently asked questions

Why do I need an anaerobic culture if I already had a regular culture?
Regular bacterial cultures often use oxygen in the incubation process, which can kill anaerobic bacteria before they are detected. An anaerobic culture specifically creates an oxygen-free environment to allow these bacteria to grow and be identified. Both tests may be ordered together to get a complete picture of what is causing your infection.
How long does it take to get results?
Results typically take 2-7 days. Anaerobic bacteria grow more slowly than aerobic bacteria, and the lab needs adequate time to allow the bacteria to multiply to a level where they can be properly identified and tested for antibiotic sensitivity.
Does this test hurt?
The discomfort depends on where the sample is collected. Drawing blood may cause brief pain similar to a regular blood test. Collecting fluid from an abscess or wound may cause some tenderness. Your healthcare provider will take steps to minimize any discomfort.
What do the results mean?
A positive result means anaerobic bacteria were found and identified, which helps explain the cause of your infection. A negative result means no anaerobic bacteria were detected, but it does not completely rule out an infection—it may mean the infection is caused by other organisms or that the sample did not contain enough bacteria to detect. Your doctor will interpret the results in context with your symptoms and other test findings.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy