Laboratory Blood Test: What You Need to Know Before Your Appointment
This test identifies anaerobic bacteria in samples from the lower respiratory tract (such as sputum or bronchoalveolar lavage) to help diagnose lung infections caused by anaerobic bacteria.
{"This test detects anaerobic bacteria that can cause lung infections","Samples are collected from the lower respiratory tract (sputum or bronchoalveolar lavage)","Results help doctors choose the right antibiotics for treatment","Anaerobic bacteria thrive in environments without oxygen","Proper sample collection is crucial for accurate results"}
Key facts at a glance
- LOINC
- 89644-9
- Short code
- RESPANAC
- Synonyms
- Bacteria Lower resp Anaerobe Cult
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, leak-proof container without preservative for sputum or bronchoscopy specimens. Anaerobic transport system (e.g., anaerobic transport medium, Port-a-Cul vial) required for tissue or aspirate specimens to maintain viability of obligate anaerobes. Transport immediately at room temperature.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–120 years
- Method
- anaerobic-culture
- CPT
- 87075
- SNOMED
- 415430008
- Specimen volume
- Minimum 1-2 mL for respiratory secretions (sputum, bronchial washings, bronchoalveolar lavage). For tissue specimens, submit representative tissue fragments in anaerobic transport medium.
- Stability
- Specimens should be transported to the laboratory within 2 hours of collection at room temperature for optimal anaerobic organism recovery. Specimens in anaerobic transport medium are stable for up to 24-48 hours at room temperature. Do not refrigerate specimens intended for anaerobic culture as cold temperature is lethal to many obligate anaerobes.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–168 hours
- Order
- routine
What is this test and what does it help your doctor learn about your health?
The Lower Respiratory Anaerobic Bacterial Culture is a laboratory test that detects and identifies anaerobic bacteria in samples collected from the lower respiratory tract. Anaerobic bacteria are microorganisms that can grow without oxygen and are known to cause various lung infections, including pneumonia, lung abscesses, and bronchitis. The test involves collecting a sample (such as sputum or bronchoalveolar lavage fluid) and culturing it in a special environment to allow anaerobic bacteria to grow and be identified.
How is the sample collected for this test, and what can you expect during the procedure?
The most common methods for collecting lower respiratory samples include: (1) Sputum collection: You will be asked to cough deeply and spit mucus from your lungs into a sterile container; (2) Bronchoalveolar lavage (BAL): A thin tube is passed through your nose or mouth into your lungs to wash out and collect fluid samples; (3) Tracheal aspirate: A sample is collected from the windpipe using a suction device. Your healthcare provider will determine the most appropriate collection method based on your condition.
Why is this test important for diagnosing conditions and guiding your treatment plan?
Anaerobic bacteria are a common cause of aspiration pneumonia, which often occurs when mouth or stomach contents are inhaled into the lungs. These infections can be severe and require specific antibiotic treatment. This test helps doctors identify the exact bacteria causing the infection, allowing them to prescribe targeted antibiotics rather than broad-spectrum medications. This approach leads to more effective treatment and helps combat antibiotic resistance.
Reference range
No anaerobic bacteria isolated (negative). Results reported as specific organism identified with colony morphology and quantification when applicable.N/A
Patient should rinse mouth with water prior to specimen collection to minimize oral contamination. For bronchoscopy procedures, patient should fast for at least 4-6 hours prior to procedure. If collecting sputum, patient should be instructed on proper deep cough technique. Prior antibiotic therapy should be documented as it may affect culture results.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Frequently asked questions
Does the test hurt?
How long does it take to get results?
Do I need to prepare for the test?
What do the results mean?
Sources
- 1
- 2
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy