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Laboratory Blood Test: What You Need to Know Before Your Appointment

Published: August 9, 2026Updated: August 16, 2026

This test identifies anaerobic bacteria in samples from the lower respiratory tract (such as sputum or bronchoalveolar lavage) to help diagnose lung infections caused by anaerobic bacteria.

Quick summary

{"This test detects anaerobic bacteria that can cause lung infections","Samples are collected from the lower respiratory tract (sputum or bronchoalveolar lavage)","Results help doctors choose the right antibiotics for treatment","Anaerobic bacteria thrive in environments without oxygen","Proper sample collection is crucial for accurate results"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
89644-9
Short code
RESPANAC
Synonyms
Bacteria Lower resp Anaerobe Cult
Category
chemistry
Sample
serum
Container
Sterile, leak-proof container without preservative for sputum or bronchoscopy specimens. Anaerobic transport system (e.g., anaerobic transport medium, Port-a-Cul vial) required for tissue or aspirate specimens to maintain viability of obligate anaerobes. Transport immediately at room temperature.
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–120 years
Method
anaerobic-culture
CPT
87075
SNOMED
415430008
Specimen volume
Minimum 1-2 mL for respiratory secretions (sputum, bronchial washings, bronchoalveolar lavage). For tissue specimens, submit representative tissue fragments in anaerobic transport medium.
Stability
Specimens should be transported to the laboratory within 2 hours of collection at room temperature for optimal anaerobic organism recovery. Specimens in anaerobic transport medium are stable for up to 24-48 hours at room temperature. Do not refrigerate specimens intended for anaerobic culture as cold temperature is lethal to many obligate anaerobes.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
48–168 hours
Order
routine

What is this test and what does it help your doctor learn about your health?

The Lower Respiratory Anaerobic Bacterial Culture is a laboratory test that detects and identifies anaerobic bacteria in samples collected from the lower respiratory tract. Anaerobic bacteria are microorganisms that can grow without oxygen and are known to cause various lung infections, including pneumonia, lung abscesses, and bronchitis. The test involves collecting a sample (such as sputum or bronchoalveolar lavage fluid) and culturing it in a special environment to allow anaerobic bacteria to grow and be identified.

How is the sample collected for this test, and what can you expect during the procedure?

The most common methods for collecting lower respiratory samples include: (1) Sputum collection: You will be asked to cough deeply and spit mucus from your lungs into a sterile container; (2) Bronchoalveolar lavage (BAL): A thin tube is passed through your nose or mouth into your lungs to wash out and collect fluid samples; (3) Tracheal aspirate: A sample is collected from the windpipe using a suction device. Your healthcare provider will determine the most appropriate collection method based on your condition.

Why is this test important for diagnosing conditions and guiding your treatment plan?

Anaerobic bacteria are a common cause of aspiration pneumonia, which often occurs when mouth or stomach contents are inhaled into the lungs. These infections can be severe and require specific antibiotic treatment. This test helps doctors identify the exact bacteria causing the infection, allowing them to prescribe targeted antibiotics rather than broad-spectrum medications. This approach leads to more effective treatment and helps combat antibiotic resistance.

Reference range

No anaerobic bacteria isolated (negative). Results reported as specific organism identified with colony morphology and quantification when applicable.N/A

Specimen: serum

Patient should rinse mouth with water prior to specimen collection to minimize oral contamination. For bronchoscopy procedures, patient should fast for at least 4-6 hours prior to procedure. If collecting sputum, patient should be instructed on proper deep cough technique. Prior antibiotic therapy should be documented as it may affect culture results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Does the test hurt?
Sputum collection is generally painless, though you may feel some discomfort when coughing deeply. Bronchoalveolar lavage is performed under local anesthesia, so you may feel pressure but not significant pain. Your healthcare team will ensure your comfort during the procedure.
How long does it take to get results?
Anaerobic bacterial culture typically takes 48-72 hours for initial results, but complete identification and antibiotic sensitivity testing may require 5-7 days. This is because anaerobic bacteria grow more slowly than aerobic bacteria.
Do I need to prepare for the test?
For sputum collection, rinse your mouth with water before collecting the sample to reduce contamination from normal mouth bacteria. Do not use mouthwash or brush your teeth immediately before collection as this may affect results. Follow any specific instructions provided by your healthcare provider.
What do the results mean?
A positive result means anaerobic bacteria were found in your respiratory sample, indicating an anaerobic bacterial infection. A negative result suggests no anaerobic bacteria were detected, though this does not completely rule out infection. Your doctor will interpret the results in conjunction with your symptoms, imaging studies, and other test results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy