Understanding Your Laboratory Test: A Complete Patient Guide to Testing, Sample Collection, and Interpreting Your Results
This test identifies and characterizes bacteria found in peritoneal (abdominal) fluid to diagnose abdominal infections.
{"A peritoneal fluid culture helps detect bacterial infections in the abdominal cavity","Results identify specific bacteria causing the infection","Results guide antibiotic treatment selection","The test is crucial for diagnosing peritonitis","Sample is collected via a procedure called paracentesis"}
Key facts at a glance
- LOINC
- 17959-8
- Short code
- PERIC2
- Synonyms
- Bacteria Prt Cult org #2
- Category
- chemistry
- Sample
- serum
- Container
- Sterile, leak-proof container without preservative; aerobic and anaerobic blood culture bottles may also be used. For larger volumes, sterile screw-cap tubes or transport swabs in Amies or Stuart transport medium are acceptable.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 0 years
- Method
- culture
- CPT
- 87077
- SNOMED
- 440271007
- Specimen volume
- 5-10 mL of peritoneal fluid recommended; minimum 1-3 mL acceptable for culture. Larger volumes improve recovery of fastidious and anaerobic organisms.
- Stability
- Specimen should be delivered to the laboratory immediately. If processing is delayed, refrigerate at 2-8°C; do not freeze. Specimens in blood culture bottles are stable at room temperature for up to 24 hours. Ideally, process within 2 hours of collection for optimal organism recovery.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 24–120 hours
- Order
- routine
This laboratory test is a diagnostic procedure in which a small sample of your blood, urine, or other body fluid is analyzed in a medical laboratory to help your healthcare provider assess your overall health, screen for certain conditions, or monitor a known medical problem.
The Peritoneal Fluid Culture test analyzes fluid collected from the abdominal cavity to identify bacteria that may be causing an infection. When bacteria are found in the fluid, they are grown in a laboratory (cultured) and identified to determine the exact type causing the infection.
The sample is usually collected at a clinic, hospital, or laboratory draw station. A trained phlebotomist will clean your skin, wrap a band around your upper arm, and draw blood from a vein, typically in your arm, using a sterile needle; some tests may instead require a urine, saliva, or swab sample that you can often provide yourself.
A healthcare provider collects peritoneal fluid using a procedure called paracentesis. A thin needle is inserted through the abdominal wall to withdraw fluid from the peritoneal cavity. The fluid is then sent to a laboratory for culture and analysis.
Your doctor orders this test to help confirm or rule out a suspected condition, check how well organs such as the kidneys, liver, or thyroid are working, evaluate symptoms you may be experiencing, or track how well a treatment plan you are already following is working.
This test is performed when a patient shows symptoms of abdominal infection such as abdominal pain, fever, or distension. It helps doctors diagnose peritonitis (inflammation of the abdominal lining) and determine the appropriate antibiotic treatment.
Your results will show whether your values fall within the normal reference range listed on your report. Values within range generally suggest typical organ function, while results that are higher or lower than expected may point to an infection, inflammation, or another condition that your provider will discuss with you, sometimes recommending follow-up testing or treatment.
A positive result means bacteria have been found in the peritoneal fluid, indicating an infection. The laboratory will identify the specific type of bacteria, which helps doctors choose the most effective antibiotics. A negative result means no bacteria were detected, though this does not completely rule out infection.
Reference range
No growth of pathogenic bacteria. Normal peritoneal fluid is sterile. Any growth of pathogenic organisms is considered clinically significant and reported with identification and susceptibility results.N/A
No special patient preparation required. Specimen should be collected prior to initiation of antimicrobial therapy whenever possible. Inform the laboratory of current or recent antibiotic use as this may affect culture results.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is primarily used to diagnose bacterial peritonitis, which can occur due to rupture of the appendix, stomach ulcer, intestinal perforation, or as a complication of peritoneal dialysis. Identifying the specific bacteria helps guide targeted antibiotic therapy and improves patient outcomes. The test is also valuable for monitoring treatment effectiveness.
Frequently asked questions
Is the test painful?
How long does it take to get results?
What happens after a positive result?
Are there any risks with paracentesis?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy