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Bacteriapuscult

Published: August 9, 2026Updated: August 16, 2026

A laboratory test that identifies and grows bacteria from a pus sample to determine what type of infection you have and which antibiotics will work best.

Quick summary

{"This test helps doctors identify the specific bacteria causing your infection","Results guide antibiotic treatment decisions","Sample is collected from an infected wound, abscess, or pus-filled area","Results typically take 2-5 days to complete","Different bacteria require different antibiotics for treatment"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
106032-6
Short code
PUSCULT
Synonyms
Bacteria Pus Cult
Category
chemistry
Sample
serum
Container
Sterile, leak-proof container without preservative; sterile swab transport system (e.g., Amies or Stuart transport medium) for swab specimens; sterile screw-capped tube or syringe for aspirated pus; anaerobic transport vial for specimens requiring anaerobic culture
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
culture
CPT
87070 (Culture, bacterial; any other source except urine, blood, or stool, with isolation and presumptive identification of isolates); 87075 (Culture, bacterial; any source, anaerobic with isolation and presumptive identification of isolates); 87076 (anaerobic identification); 87077 (aerobic identification); 87186 (susceptibility, MIC); 87147 (susceptibility, disk method); 87205 (Gram stain)
SNOMED
43896003 (Bacterial culture of pus)
Specimen volume
Pus: 1-5 mL preferred (minimum 0.5 mL); Swab: one or two swabs from the same site placed in transport medium; Aspirate: as much volume as clinically obtainable
Stability
Room temperature (20-25°C): process within 2 hours for optimal organism viability. If delayed, refrigerate at 2-8°C for up to 24 hours. For anaerobic culture specimens, maintain at room temperature and process as soon as possible. Specimens in anaerobic transport medium are stable at room temperature for up to 48 hours per manufacturer's instructions.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
48–168 hours
Order
routine

What is this test?

A pus culture, also called a bacterial culture of pus, is a laboratory test that takes a sample of pus from an infected area and places it in a special environment to grow and identify any bacteria present. This helps your doctor understand exactly what is causing your infection.

Why is this test done?

Your doctor may order this test if you have an infected wound, abscess, or other pus-filled area. The results help determine the exact type of bacteria causing the infection, which allows your doctor to prescribe the most effective antibiotic for your specific condition.

How is the sample collected?

A healthcare provider will clean the infected area and collect a small sample of pus using a sterile swab or needle. The sample is then placed in a sterile container and sent to the laboratory for analysis.

What do the results mean?

If bacteria grow in the culture, the lab will identify the specific type and perform additional tests (called susceptibility or sensitivity testing) to see which antibiotics can effectively kill the bacteria. If no bacteria grow, it could mean the infection is caused by viruses, fungi, or that the sample was collected improperly.

Reference range

No growth of pathogenic bacteria. Reportable organisms include Staphylococcus aureus, Streptococcus pyogenes, Streptococcus agalactiae, Enterobacteriaceae, Pseudomonas aeruginosa, anaerobes, and other clinically significant isolates. Normal skin flora (e.g., coagulase-negative staphylococci, Corynebacterium species, Cutibacterium acnes) may be reported as normal flora when not clinically significant.N/A

Specimen: serum

No fasting required. Patient should not apply topical antibiotics, antiseptics, or dressings to the affected area for at least 24 hours prior to specimen collection, if clinically feasible. The collection site should be cleaned with sterile saline or 70% alcohol before sampling to reduce contamination with surface flora. For deep wound or abscess specimens, prefer aspirated pus over swab specimens when possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long does it take to get results?
Results typically take 2-5 days. Bacteria need time to grow in the laboratory, and additional tests to check antibiotic sensitivity may take extra time.
Does the test hurt?
You may feel some discomfort when the sample is collected, similar to a minor injection. The area will be cleaned before sampling to minimize pain.
What happens if no bacteria grow in the culture?
If no bacteria grow, it could mean the infection is caused by viruses or fungi, the infection is responding to treatment, or the sample did not contain enough bacteria. Your doctor will discuss next steps based on your symptoms.
Do I need to prepare for this test?
No special preparation is needed. Just keep the infected area clean and follow your doctor's instructions about caring for the wound before your appointment.
Will I need antibiotics after this test?
Your doctor will determine if antibiotics are needed based on your infection and culture results. If bacteria are found, the most effective antibiotic will be prescribed. Sometimes treatment may start before results are available and then adjusted based on findings.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy