Understanding Your Laboratory Test: What It Is, How It Is Performed, and How to Get Ready
A wound culture test identifies aerobic bacteria present in a shallow wound to help guide appropriate treatment.
{"This test detects and identifies bacteria in wound tissue","Results help doctors choose the right antibiotics","Sample is collected from the wound using a swab or tissue","Results typically available in 2-5 days","Proper wound care is essential alongside this test"}
Key facts at a glance
- LOINC
- 17920-0
- Short code
- BCWND6
- Synonyms
- Bacteria Shlw Wnd Aerobe Cult org #6
- Category
- chemistry
- Sample
- serum
- Container
- Sterile swab transport system (e.g., Amies or Stuart's medium) or sterile screw-cap container for aspirates/tissue; culturette transport swab
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 0 years
- Method
- aerobic-culture
- CPT
- 87077
- SNOMED
- 395196007
- Specimen volume
- Minimum: one swab or 0.5-1 mL of fluid; tissue specimens should be at least 1-2 cm³ when possible
- Stability
- Room temperature: 24 hours; Refrigerated (2-8°C): 48 hours. Specimens should be transported to the laboratory as soon as possible after collection to maximize organism viability.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–120 hours
- Order
- routine
This test is a laboratory analysis that helps your doctor evaluate your overall health, look for signs of disease, or monitor a condition you already have. Your results are compared with established reference ranges so your care team can make informed decisions about your diagnosis and treatment.
Aerobic wound culture is a laboratory test that detects and identifies aerobic bacteria living in a shallow wound. Aerobic bacteria require oxygen to grow and are common causes of wound infections. The test involves collecting a sample from the wound surface or tissue and placing it in conditions that allow bacteria to grow and be identified.
A trained health professional will collect your sample, most often a small amount of blood drawn from a vein in your arm using a sterile needle. Depending on the test, you may instead be asked to provide a urine sample, a throat or nasal swab, or another specimen in a clean container.
A healthcare provider will clean the wound with sterile saline, then collect a sample using a sterile swab, tissue, or fluid from the wound area. The sample is then placed in a transport container and sent to the laboratory for analysis. Proper collection technique is important to avoid contamination.
Once your sample reaches the laboratory, trained technologists label it, prepare it, and analyze it using specialized instruments and validated methods. The results are carefully reviewed for accuracy, entered into your medical record, and sent to your doctor, who will discuss the meaning with you.
In the lab, the sample is placed on growth media and incubated to allow aerobic bacteria to multiply. Technicians then identify the specific types of bacteria present and may perform antibiotic sensitivity testing (susceptibility testing) to determine which antibiotics will be effective against the identified bacteria.
Preparation depends on the specific test your doctor has ordered. You may be asked to fast for a certain number of hours, avoid certain foods or medicines, or drink plenty of water beforehand. Always follow the instructions you receive, and tell the staff about any medicines or supplements you take.
No special preparation is needed for this test. Simply keep the wound clean and dry before your appointment. Inform your healthcare provider about any antibiotics you are currently taking as this may affect results.
Reference range
No aerobic bacteria isolated after standard incubation period. Normal skin flora may be reported when clinically relevant.N/A
No fasting required. Ideally, the patient should not have received topical or systemic antibiotics for at least 48-72 hours prior to specimen collection when clinically feasible. The wound area should not be cleansed with antiseptics immediately before sampling unless debridement is performed first.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Wound culture results help healthcare providers diagnose wound infections and select appropriate antibiotic treatment. Identifying the specific bacteria allows for targeted therapy rather than using broad-spectrum antibiotics, which helps combat antibiotic resistance. Common wound pathogens include Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa. Negative results may indicate that the wound is healing well without bacterial infection or that the infection is caused by anaerobic bacteria requiring different testing.
Frequently asked questions
Does a positive culture mean I have an infection?
How long does it take to get results?
Will the test hurt?
What if no bacteria are found?
Sources
- 1
- 2
- 3LOINC - 17920-0Source
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy