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Your Guide to This Test: What It Is, How It Is Performed, and What Your Results Mean

Published: August 9, 2026Updated: August 16, 2026

This test identifies specific types of anaerobic bacteria (bacteria that grow without oxygen) in a specimen collected from your body.

Quick summary

{"The test detects anaerobic bacteria that can cause infections in deep tissues","A specimen is collected and sent to a lab where bacteria are grown in special conditions without oxygen","Results help doctors choose the right antibiotic treatment","Results typically take 2-7 days as bacteria need time to grow"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
44857-1
Short code
BAC5AN
Synonyms
Bacteria Spec Anaerobe Cult org #5
Category
chemistry
Sample
serum
Container
Anaerobic transport system (e.g., Anaerobic Transport Medium, Port-A-Cul vial, or ESwab in anaerobic transport)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
anaerobic-culture
CPT
87076
SNOMED
42445006
Specimen volume
Minimum 1 mL of fluid specimen or adequate tissue sample; submit in appropriate anaerobic transport container
Stability
Room temperature: 2-4 hours in anaerobic transport medium; Refrigerated: 24-48 hours (not recommended for most specimens); Specimens should be processed as soon as possible to maintain viability of anaerobic organisms
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
48–240 hours
Order
routine

What Is This Test, and What Does It Measure in My Body?

The Anaerobic Bacteria Culture test is a laboratory test that looks for anaerobic bacteria in a sample taken from your body. Anaerobic bacteria are microorganisms that can survive and multiply in environments without oxygen. Some anaerobic bacteria are harmless, while others can cause serious infections in deep tissues, wounds, or internal organs.

How Is This Test Performed, and What Should I Expect During the Procedure?

Your healthcare provider will collect a specimen from the affected area. This could be a tissue sample, fluid from a wound, blood, or another type of sample depending on your symptoms. The specimen is placed in a special container that prevents oxygen exposure and sent to the laboratory. In the lab, scientists place the specimen in an environment without oxygen to encourage anaerobic bacteria to grow. Once the bacteria grow, they can be identified through various testing methods.

Why Do I Need This Test, and How Will It Help My Doctor Care for Me?

Your doctor may order this test if you have symptoms of an infection that might be caused by anaerobic bacteria. These infections often occur after surgery, in deep wounds, or in areas of the body with poor blood supply. Symptoms may include fever, pain, swelling, or discharge from a wound that doesn't heal properly.

What Do My Test Results Mean, and How Will My Doctor Explain Them to Me?

If anaerobic bacteria are identified, it means you have an anaerobic bacterial infection. The report will specify which type of bacteria was found. This information helps your doctor prescribe the most appropriate antibiotic, as some antibiotics are more effective against anaerobic bacteria than others. If no bacteria are grown, it could mean the infection is caused by something else or that the sample was collected improperly.

Reference range

No anaerobic growth; No anaerobic bacteria isolatedN/A

Specimen: serum

No special patient preparation required. If possible, specimen should be collected before initiation of antimicrobial therapy. Discontinue topical antibiotics or antiseptics at the collection site for 24-48 hours prior to collection when applicable.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Anaerobic bacterial infections can occur in various body sites, including the abdomen, pelvis, lungs, and soft tissues. These infections are often polymicrobial (involving multiple types of bacteria) and can lead to serious complications if left untreated. Common anaerobic pathogens include Bacteroides, Clostridium, and Prevotella species. Proper identification through culture guides targeted antibiotic therapy and helps prevent treatment failure or recurrence.

Frequently asked questions

How long does it take to get results?
Results typically take 2 to 7 days. Anaerobic bacteria grow more slowly than aerobic bacteria, and additional time may be needed to identify the specific type and determine which antibiotics will be most effective.
Does the test hurt?
The discomfort depends on where the specimen is collected. Your healthcare provider will use appropriate techniques to minimize any pain. The laboratory testing itself does not cause any pain as it is done on the collected sample.
Do I need to prepare for the test?
Your doctor will give you specific instructions based on the type of specimen needed. In general, you should inform your doctor about any antibiotics you are currently taking, as they may affect the test results.
What happens after the results?
Your doctor will discuss the results with you and determine the best treatment plan. If bacteria are identified, you may be prescribed specific antibiotics that are effective against anaerobic bacteria. Follow-up testing may be needed to ensure the infection is clearing.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy