Understanding Your Laboratory Test: What It Is and Why Your Doctor Ordered It
This test identifies anaerobic (oxygen-free) bacteria growing in a surgical wound to help diagnose and treat infections.
{"The test finds bacteria that thrive without oxygen in wound infections","Results help doctors choose the right antibiotic treatment","Proper sample collection is important for accurate results","Treatment may take several weeks depending on the bacteria found","Follow-up testing may be needed to confirm healing"}
Key facts at a glance
- LOINC
- 105933-6
- Short code
- ANSCW
- Synonyms
- Bacteria Surgical wound Anaerobe Cult
- Category
- chemistry
- Sample
- serum
- Container
- Sterile anaerobic transport swab (Amies or Stuart's medium without charcoal) or sterile screw-cap container for tissue/aspirate
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- ≥ 0 years
- Method
- anaerobic-culture
- CPT
- 87075
- SNOMED
- 442126008
- Specimen volume
- Minimum 0.5 mL of pus or aspirate; tissue specimen should be at least 1 cm³ when possible
- Stability
- Room temperature: 2 hours; refrigerated (2-8°C): up to 24 hours for swabs in transport medium; tissues/fluids should be transported immediately under anaerobic conditions
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–240 hours
- Order
- routine
This test analyzes a sample of your blood, urine, or other body fluid to help your doctor check how your body is working, look for signs of illness, screen for certain conditions, or monitor treatment you are already receiving. Your results are compared with established reference ranges, and your doctor will interpret them together with your symptoms, medical history, and physical examination to decide on the best next steps for your care.
This test looks for anaerobic bacteria in a sample taken from a surgical wound. Anaerobic bacteria are microorganisms that can grow and multiply without oxygen. These bacteria often cause deep tissue infections and can be harder to treat than regular infections because they may not respond to common antibiotics.
A trained healthcare professional will collect your sample, most often by drawing a small amount of blood from a vein in your arm using a thin needle. A band is placed around your upper arm, the site is cleaned with an antiseptic wipe, and the needle stays in place for only a few seconds. Some tests require a urine, saliva, or swab sample instead, and you will receive simple step-by-step instructions for collecting it correctly at home or in the office.
A healthcare provider will collect tissue fluid or tissue samples from the wound using a sterile swab or needle. The sample must be placed in a special container that prevents oxygen exposure, as anaerobic bacteria die when exposed to air. Proper collection is essential for accurate results.
Once your sample arrives at the laboratory, trained technologists label it, prepare it, and analyze it using specialized equipment that measures the substances your doctor wants to evaluate. Quality-control procedures are performed throughout testing to make sure results are accurate and reliable. When the analysis is complete, a laboratory professional reviews the findings, and your results are sent securely to your ordering provider, who will contact you to explain what they mean.
The sample is placed in an environment without oxygen to encourage anaerobic bacteria to grow. Laboratory technicians then identify the specific types of bacteria present and test which antibiotics can effectively treat the infection. This process usually takes 3-7 days.
Reference range
No growth of anaerobic pathogens. Report includes isolated organism(s), Gram stain characteristics, and quantity (rare, few, moderate, many)N/A
No fasting required. Discontinue topical antibiotics or antimicrobial wound dressings at least 24-48 hours prior to specimen collection if clinically feasible. Avoid contamination with skin flora during collection
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Identifying the specific anaerobic bacteria causing the wound infection helps doctors select the most effective antibiotic treatment. Anaerobic wound infections can lead to serious complications if untreated, including tissue death (gangrene) and spread of infection to the bloodstream. This test is particularly important for deep surgical wounds, diabetic foot ulcers, and bite wounds.
Common anaerobic bacteria found in wound infections include Bacteroides, Clostridium, and Peptostreptococcus species. The test results guide targeted therapy rather than using broad-spectrum antibiotics, which helps reduce antibiotic resistance and side effects.
Frequently asked questions
Does the test hurt?
How long does it take to get results?
Do I need to prepare for this test?
What if the test is negative?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy