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Bacteriaurcultorg2

Published: August 9, 2026Updated: August 16, 2026

This test identifies and analyzes the second type of bacteria found in your urine culture to help diagnose urinary tract infections.

Quick summary

{"A urine culture tests for bacteria in your urine that may be causing a urinary tract infection (UTI)","When multiple bacteria are found, each is identified and numbered for proper analysis","The second bacteria identified helps doctors understand complex infections and choose the right treatment","Results help determine if one or more antibiotics are needed to treat the infection","This test is part of a complete urine culture analysis ordered by your healthcare provider"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17970-5
Short code
UCULT2
Synonyms
Bacteria Ur Cult org #2
Category
chemistry
Sample
urine
Container
Sterile, wide-mouth, leak-proof urine container with screw-cap lid; boric acid preservative tubes may be used for transport; BD Vacutainer gray-top or equivalent preservative tube acceptable
Units
N/A
Ref low
0
Ref high
100
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
culture
CPT
87086
SNOMED
165811005
Specimen volume
10-50 mL for routine culture; minimum 1-3 mL acceptable for pediatric or catheterized specimens
Stability
Unpreserved urine: room temperature ≤2 hours; refrigerated (2-8°C) ≤24 hours. Preserved urine with boric acid: room temperature ≤48 hours; refrigerated ≤72 hours. Specimens should be processed as soon as possible to ensure accurate bacterial identification.
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this test?

A urine bacteria culture is a laboratory test that detects and identifies bacteria in urine. When multiple types of bacteria are found in the culture, each is assigned a number (such as 'Bacteria #1', 'Bacteria #2') based on the order they were isolated. This test specifically identifies the second bacterium found in your urine sample.

Why is it ordered?

This test is typically ordered when a urine culture reveals more than one type of bacteria, which can happen in complicated urinary tract infections, in patients with certain risk factors, or when initial treatment has failed. Identifying all bacteria present helps your doctor understand the full picture of your infection.

How is the test performed?

A urine sample is collected (usually clean-catch midstream) and sent to a laboratory. The sample is placed in special growth media to allow bacteria to multiply. If bacteria grow, they are identified using various laboratory techniques, and each distinct type is assigned a number. The second bacterium identified undergoes the same detailed analysis as the first.

What do the results mean?

The results will show which type of bacteria was identified as the second most abundant or second isolated. Your healthcare provider will interpret these results along with your symptoms, other test results, and clinical findings to determine the appropriate treatment plan.

Reference range

No growth of clinically significant bacteria is considered normal. For clean-catch midstream urine: <10,000 CFU/mL typically considered negative or contaminants; 10,000-100,000 CFU/mL equivocal (repeat recommended); >100,000 CFU/mL suggestive of significant bacteriuria. Identification of bacteria is reported as organism name with colony count.N/A

Specimen: urine

Patient should be provided with proper instructions for clean-catch midstream urine collection. The periurethral area should be cleaned with antiseptic wipes prior to collection. First morning void is preferred as bacteria have had time to multiply, increasing sensitivity of detection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Does finding two bacteria mean I have two separate infections?
Not necessarily. Multiple bacteria can be present in a single infection, especially in complicated urinary tract infections. Your doctor will consider your symptoms and clinical history to determine the significance of each bacteria found.
Do I need to do anything special to prepare for this test?
You should provide a clean-catch urine sample. This involves cleaning the genital area before collection and catching the middle portion of the urine stream in a sterile container. Your healthcare provider will give you specific instructions.
What happens after the bacteria are identified?
Once the bacteria are identified, the laboratory may perform antibiotic susceptibility testing (sensitivity testing) to determine which antibiotics will be effective against the bacteria. This helps your doctor prescribe the most appropriate treatment.
Is it concerning to have multiple bacteria in urine?
Your healthcare provider will determine if the findings are clinically significant. Sometimes multiple bacteria may represent sample contamination, while in other cases it indicates a more complex infection requiring careful treatment. Follow your doctor's guidance based on your specific situation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy