Laboratory Blood Test: What to Expect Before, During, and After Your Sample Is Collected
A laboratory test that identifies and characterizes bacteria present in a wound to help guide appropriate antibiotic treatment.
{"This test identifies specific bacteria causing a wound infection","Results help doctors choose the most effective antibiotic","Sample is collected from the wound using a swab or tissue","Results typically take 2-5 days to complete","Accurate identification is crucial for proper treatment"}
Key facts at a glance
- LOINC
- 6462-6
- Short code
- WCULT
- Synonyms
- Bacteria Wnd Cult
- Category
- chemistry
- Sample
- serum
- Container
- Sterile transport swab (Amies or Stuart transport medium) or sterile leak-proof container for tissue/fluid aspirates. Anaerobic transport vial required for specimens suspected of containing anaerobic organisms.
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–120 years
- Method
- culture
- CPT
- 87070
- SNOMED
- 103141002
- Specimen volume
- Adequate specimen is essential. For swabs: one or two swabs in transport medium. For tissue: 1-2 cm³ of viable tissue. For aspirates: 1-5 mL of purulent material or fluid.
- Stability
- Room temperature: 2 hours maximum. If transport is delayed, refrigerate at 2-8°C (do not freeze) for up to 24 hours. Anaerobic specimens should be processed within 1-2 hours of collection.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 48–168 hours
- Order
- routine
A laboratory test is an analysis performed on a sample of your blood, urine, or other body material to help your healthcare provider understand what is happening inside your body. The results can confirm that an organ is working properly, detect a disease in its early stages, or show how well a current treatment is responding to therapy.
A wound bacterial culture test is a laboratory procedure that collects a sample from your wound (such as a cut, surgical incision, or skin sore) to identify the specific type of bacteria causing an infection. The sample is placed in a special environment that allows bacteria to grow, and then laboratory technicians analyze the grown bacteria to determine exactly what organisms are present.
Most laboratory tests use a small blood sample drawn from a vein in your arm by a trained phlebotomist. The site is cleaned, a needle collects the blood into sealed tubes, and a bandage is applied afterward; the whole process usually takes less than ten minutes. Some tests instead require a urine sample or a simple finger stick.
A healthcare professional will gently clean the wound and then use a sterile swab or collect a small tissue sample from the infected area. This sample is immediately placed in a sterile container and sent to the laboratory for analysis. The collection process is usually quick and may cause mild discomfort.
Once your sample reaches the laboratory, it is labeled with your identification details, logged into the tracking system, and analyzed by specialized instruments and trained technologists. The machines measure the components of interest, a technologist verifies the findings, and the final report is sent to your doctor, typically within a few days.
In the lab, the sample is cultured (placed in conditions that allow bacteria to grow). Once bacteria multiply, technicians can identify the specific species through various methods including visual examination, biochemical tests, and sometimes genetic testing. The lab may also perform antibiotic sensitivity testing (susceptibility testing) to determine which antibiotics will effectively treat the infection.
Your doctor will tell you whether your specific test requires fasting, which usually means avoiding all food and drinks except water for 8 to 12 hours before the draw. Stay well hydrated, keep taking your regular medications unless instructed otherwise, and mention any supplements you use, since some of them can affect your results.
No special preparation is needed for this test. Simply keep your wound clean and follow any instructions from your healthcare provider. Inform your doctor about any antibiotics you are currently taking, as this may affect the test results.
Reference range
No growth of pathogenic bacteria. Results reported as organism identification (genus and species) with antimicrobial susceptibility testing when clinically indicated.N/A
No special patient preparation is required prior to specimen collection. If patient is on antimicrobial therapy, this information should be communicated to the laboratory as it may affect culture results.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is crucial for diagnosing wound infections and determining the most appropriate treatment. By identifying the specific bacteria causing the infection, doctors can prescribe targeted antibiotics rather than using broad-spectrum antibiotics, which helps combat antibiotic resistance. The antibiotic sensitivity portion of the test ensures that the prescribed treatment will be effective against the specific bacteria present in your wound. This test is particularly important for deep or chronic wounds, surgical site infections, and wounds that are not healing properly with initial treatment.
Frequently asked questions
How long does it take to get results?
Does a positive result always mean I have an infection?
Will the test be painful?
What if no bacteria grow in the culture?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy