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Understanding This Test: What It Is, How It Is Done, and What Your Results Mean for Your Health

Published: August 9, 2026Updated: August 17, 2026

This test checks whether barbiturates, a type of sedative medication, are present in your urine sample.

Quick summary

{"Detects the presence of barbiturate drugs in your urine","Used to monitor prescribed medication use or screen for drug abuse","Results are reported as either Positive (found) or Negative (not found)","Barbiturates are rarely prescribed today due to safer alternatives"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
3377-9
Short code
BARBU
Synonyms
Barbiturates Ur Ql
Category
chemistry
Sample
urine
Container
Sterile, leak-proof urine container without preservative
Units
N/A
Ref low
3.5
Ref high
7.2
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
Immunoassay (screening) with confirmation by Gas Chromatography-Mass Spectrometry (GC-MS)
CPT
80307
SNOMED
33359004
Specimen volume
30-50 mL random urine
Stability
Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days; Room temperature: 24 hours
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–72 hours
Order
routine

This test is a medical evaluation that helps your healthcare provider check how your body is working and look for signs of illness or other health conditions. Your doctor may order it as part of a routine checkup, to help diagnose a specific concern, or to monitor a condition you already have.

The urine barbiturate test is a screening test that detects whether barbiturates are present in your urine. Barbiturates are a class of prescription sedative medications that were historically used to treat anxiety, insomnia, and seizure disorders. Today, they are rarely prescribed because safer alternatives (such as benzodiazepines) are available.

The test is performed by a trained member of your care team, usually in a doctor's office, clinic, or hospital. Depending on the type of test, it may involve giving a small sample, such as blood or urine, or having a simple measurement or scan. The procedure is typically quick, and you can usually return to your normal activities right afterward.

You will be asked to provide a urine sample in a sterile container. The sample is then sent to a laboratory where it is analyzed using specialized equipment to detect the presence of barbiturates or their metabolites. No special preparation is typically required, though you should inform your healthcare provider about any prescription medications, over-the-counter drugs, or supplements you are taking.

Your results show the measurements or findings from the test, and your healthcare provider will review them with you. Values within the expected range generally suggest typical body function, while values outside that range may point to a condition that needs closer attention. Always talk with your provider about what your specific results mean for you.

Your test results will typically be reported as either Positive or Negative: A Positive result means barbiturates were detected in your urine. A Negative result means no barbiturates were detected at the testing threshold. It is important to discuss your results with your healthcare provider, who will interpret them in the context of your medical history and current medications.

Reference range

NCBI: Uric Acid Male 3.5-7.2 mg/dL; Female 2.5-6.8 mg/dLN/A

Specimen: urine

No fasting required. Patient should be informed about the test purpose. Collect sample under chain-of-custody procedures if medico-legal testing is required. Avoid contamination during collection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings2

Frequently asked questions

Why would my doctor order this test?
Your doctor may order this test if you are currently prescribed a barbiturate medication to ensure you are taking it properly, if you are being screened for potential drug abuse, or if you are experiencing symptoms that could be related to barbiturate use or overdose.
How long do barbiturates stay in urine?
The detection window varies depending on the specific barbiturate, the dose, and individual factors such as metabolism and kidney function. Generally, barbiturates can be detected in urine for several days to a few weeks after last use. Short-acting barbiturates like pentobarbital may be detectable for 2-4 days, while long-acting ones like phenobarbital may be detectable for up to 2-3 weeks.
Can anything else cause a positive result?
Certain medications, supplements, or foods are not expected to cause false positive results for barbiturate testing. However, it is important to inform your healthcare provider about all medications you are taking, including prescription drugs, over-the-counter medications, and herbal supplements, as these may need to be considered when interpreting your results.
Is this test the same as a drug test for employment?
This test may be part of a broader drug screening panel used for employment purposes. However, standard employment drug panels typically test for commonly abused substances like amphetamines, cocaine, opioids, and marijuana. Barbiturate testing is more commonly ordered in specific clinical or legal contexts.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy