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Understanding Your Laboratory Test: A Patient Guide to What It Is, Why It Is Done, and What to Expect

Published: August 9, 2026Updated: August 16, 2026

This test counts basophils (a type of white blood cell) in the cerebrospinal fluid (CSF) using manual microscopic examination.

Quick summary

{"The test examines cerebrospinal fluid for basophil cells","Basophils are rare in normal CSF but may increase with certain conditions","The test helps diagnose infections, inflammatory conditions, or cancers affecting the brain and spinal cord","Sample is collected via lumbar puncture (spinal tap)","Results are interpreted by comparing to normal reference values"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
55771-0
Short code
CSF BASO
Synonyms
Basophils CSF Manual
Category
chemistry
Sample
csf
Container
Sterile CSF collection tube (polypropylene or polystyrene); tube #3 or #4 typically preferred for cell count to minimize blood contamination from traumatic tap
Units
N/A
Ref low
0
Ref high
0
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
manual-count
CPT
89051
SNOMED
442980004
Specimen volume
1-2 mL of CSF sufficient for manual cell count; minimum 0.5 mL for accurate basophil identification
Stability
CSF should be analyzed within 1 hour of collection for optimal cell morphology preservation; if delayed, refrigerate at 2-8°C and analyze within 4 hours. Do not freeze.
Hemolysis max
200
TAT
1–4 hours
Order
routine

This test is a laboratory analysis that helps your healthcare provider evaluate your health. It examines a sample in a clinical lab to measure specific substances, cells, or markers in your body, giving important information about how your organs and systems are working and supporting an accurate diagnosis.

The CSF Basophil Count is a laboratory test that counts the number of basophils in the cerebrospinal fluid (CSF). Basophils are a type of white blood cell that play a role in the immune response, particularly in allergic reactions and inflammation. This test is performed manually under a microscope by a trained laboratory technologist.

Your doctor may order this test to help diagnose a medical condition, monitor an ongoing illness, check how well a treatment is working, or screen for potential health problems before symptoms appear. The results provide valuable information that guides decisions about your ongoing care and treatment plan.

This test is ordered when a doctor suspects certain conditions affecting the central nervous system. It helps detect infections, inflammatory conditions, or cancers involving the brain and spinal cord. The test may be part of a broader CSF analysis called a CSF cell count with differential.

A trained professional will collect the sample, often a small amount of blood drawn from a vein in your arm using a thin needle, though some tests use urine, saliva, or a swab instead. The collection usually takes only a few minutes, and the sample is then labeled and sent to a laboratory for analysis.

The sample is collected through a procedure called a lumbar puncture or spinal tap. A healthcare provider inserts a thin needle into the lower spine to withdraw cerebrospinal fluid. The procedure is usually performed while you lie on your side or sit bent forward.

This test is generally very safe. You may feel a quick sting or slight discomfort when the needle is inserted, and minor bruising or soreness at the site can occur afterward. Serious complications such as infection or excessive bleeding are rare. Tell your provider if you take blood thinners or have a bleeding disorder.

Lumbar puncture is generally safe, but some people may experience headache, back pain, or rarely, infection or bleeding. Your healthcare provider will discuss the risks and benefits with you before the procedure.

Reference range

Normally absent or extremely rare in healthy cerebrospinal fluid. Basophils are not typically reported in standard CSF reference ranges as their presence is generally considered pathological when observed.N/A

Specimen: csf

No fasting required. Patient should remain still during lumbar puncture. Standard aseptic lumbar puncture procedure should be performed by qualified personnel.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated basophils in CSF may indicate: allergic reactions affecting the central nervous system, certain infections (such as tuberculosis or viral meningitis), inflammatory conditions like multiple sclerosis, or rarely, leukemia or other cancers involving the central nervous system. Normal CSF typically has very few or no basophils. The presence of increased basophils, along with other CSF findings, helps doctors diagnose and differentiate various neurological conditions.

Frequently asked questions

Is basophil presence in CSF normal?
Basophils are normally absent or present in very low numbers in cerebrospinal fluid. Even a small increase may be clinically significant and requires further evaluation.
How long does it take to get results?
Results are typically available within 1-2 days, as the test requires manual microscopic examination by trained personnel.
Do I need to prepare for the lumbar puncture?
Your doctor will provide specific instructions. You may need to stop certain medications. It's important to inform your doctor about any blood-thinning medications or bleeding disorders.
Can this test diagnose specific conditions?
This test alone cannot diagnose a specific condition. It provides important information when combined with other CSF tests (like glucose, protein, and other cell counts) and your clinical symptoms. Your doctor will interpret all findings together.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy