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Basophilsnframnmanual

Published: August 9, 2026Updated: August 16, 2026

A laboratory test that counts basophils (a type of white blood cell) in the amniotic fluid surrounding your baby during pregnancy.

Quick summary

{"The test checks for white blood cells called basophils in the amniotic fluid","It helps doctors assess fetal health and detect potential infections or inflammation","A small sample of amniotic fluid is collected and examined under a microscope","Results can indicate if the baby's lungs are mature enough for birth","The test is typically performed in the later stages of pregnancy"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
38184-8
Short code
BASOLKAF
Synonyms
Basophils NFr Amn Manual
Category
chemistry
Sample
fluid
Container
Sterile, screw-cap, leak-proof polypropylene tube; sterile glass container acceptable
Units
N/A
Ref low
0
Ref high
1
Critical range
— – 10 N/A
Fasting
false
Population
adult
Sex
female
Age range
15–55 years
Method
manual-count
CPT
88108
SNOMED
409480009
Specimen volume
5-10 mL of amniotic fluid (minimum 3 mL required for manual cell count)
Stability
Room temperature: 1 hour; Refrigerated (2-8°C): 4 hours; Do not freeze. Process promptly to prevent cell degeneration.
Hemolysis max
300
TAT
2–24 hours
Order
routine

What is this test?

The Basophils/Leukocytes in Amniotic fluid test is a laboratory examination that counts the number of basophils (a type of white blood cell) in the amniotic fluid. Amniotic fluid is the protective liquid that surrounds your growing baby in the uterus during pregnancy. This test is performed manually by trained laboratory technicians who examine the fluid sample under a microscope.

How is the test performed?

A sample of amniotic fluid is collected through a procedure called amniocentesis, where a thin needle is inserted through the abdomen into the uterus to withdraw a small amount of fluid. This sample is then sent to the laboratory, where technicians stain the fluid and count the basophils and other white blood cells under a microscope. The results are reported as a count or percentage of white blood cells present.

Why might I need this test?

Your healthcare provider may order this test to evaluate fetal lung maturity, detect signs of infection or inflammation in the amniotic cavity, or assess overall fetal well-being. It may be recommended if there are concerns about preterm labor, suspected intrauterine infection, or when determining the best timing for delivery.

Reference range

Basophils are typically absent or rarely present (<1%) in normal amniotic fluid. Leukocyte counts in normal amniotic fluid are generally low (<100 cells/μL). Elevated basophil or leukocyte counts may indicate intra-amniotic infection or inflammation.N/A

Specimen: fluid

Amniocentesis performed under sterile conditions by qualified obstetrician. Patient should be informed of procedure risks including infection, membrane rupture, and fetal loss.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the amniocentesis procedure safe?
Amniocentesis carries a small risk of complications, including infection, bleeding, or miscarriage. The procedure is typically performed under ultrasound guidance to minimize risks. Your healthcare provider will discuss the benefits and risks with you before the procedure.
How long does it take to get results?
Results from a manual basophil count typically take 1-3 days, as the test requires manual microscopic examination by trained personnel. Your healthcare provider will contact you with the results and explain what they mean for your pregnancy.
What do abnormal results mean?
Elevated basophil counts in amniotic fluid may indicate intrauterine infection, inflammation, or other complications. However, your healthcare provider will interpret the results in context with other clinical findings and tests to determine the appropriate course of action.
Does this test tell me about my baby's lung maturity?
While basophil count alone is not a direct measure of lung maturity, it is often part of a broader assessment. Other tests, such as the lecithin-sphingomyelin (L/S) ratio, are more commonly used to evaluate fetal lung maturity. Your doctor will use multiple factors to determine if your baby's lungs are developed enough for delivery.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy