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Basophilsnfrprtmanual

Published: August 9, 2026Updated: August 16, 2026

This test measures the number of basophils (a type of white blood cell) in fluid collected from the abdominal cavity.

Quick summary

{"Basophils are white blood cells that help fight infections and respond to allergens","This test examines fluid from the abdominal cavity (peritoneal fluid)","Abnormal basophil counts may indicate infection, inflammation, or other conditions","The test is performed manually under a microscope by a lab technician","Results help doctors diagnose the cause of abdominal fluid buildup"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
31996-2
Short code
PERITBASO
Synonyms
Basophils NFr Prt Manual
Category
chemistry
Sample
serum
Container
Sterile, leak-proof collection container (e.g., EDTA tube for cell count and differential; plain sterile tube for additional studies); heparinized tube acceptable for cell preservation
Units
N/A
Ref low
0
Ref high
0.5
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
manual-count
CPT
89051
SNOMED
127910008
Specimen volume
5-10 mL of peritoneal fluid (minimum 1-2 mL for manual cell count)
Stability
Refrigerate at 2-8°C; analyze within 1-2 hours of collection for optimal cell morphology. EDTA specimens stable up to 24 hours refrigerated.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
1–4 hours
Order
routine

What is this test?

This test counts the number of basophils in peritoneal fluid. Peritoneal fluid is the fluid that naturally exists in the abdominal cavity. When too much fluid accumulates (a condition called ascites), doctors may need to analyze it to find out what is causing the buildup.

What are basophils?

Basophils are a type of white blood cell (leukocyte). They are part of your immune system and play important roles in: fighting infections by attacking foreign substances, responding to allergic reactions, and participating in inflammatory responses. While basophils make up only a small percentage of your white blood cells, they are important for protecting your health.

How is the test performed?

A sample of peritoneal fluid is collected through a procedure called paracentesis (also known as an abdominal tap). During this procedure, a thin needle is inserted into the abdominal cavity to withdraw fluid. In the laboratory, a technician examines the fluid under a microscope and manually counts the basophils among all the white blood cells present.

Why might I need this test?

This test is usually ordered when you have fluid buildup in your abdomen. It helps your doctor determine the cause of the fluid accumulation, which could be due to conditions such as liver disease, heart failure, infection, cancer, or pancreatitis.

Reference range

Basophils/Leukocytes ratio in peritoneal fluid is typically 0%; basophils are rarely present in normal peritoneal fluid. Reference values should be interpreted in conjunction with total leukocyte count and clinical presentation.N/A

Specimen: serum

No special patient preparation required. Peritoneal fluid is obtained via paracentesis (abdominocentesis) performed by a qualified healthcare provider under sterile conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the paracentesis procedure painful?
You will receive a local anesthetic to numb the area, so you should feel minimal discomfort during the procedure. You may feel some pressure or mild pain when the needle is inserted. After the procedure, you may have some soreness at the injection site.
How long does it take to get results?
Results are typically available within 1-2 days, as the manual cell counting process takes time to complete accurately.
Are there any risks from the paracentesis?
Minor risks include bruising, bleeding, or infection at the puncture site. Rare complications may include leakage of fluid from the site or injury to organs in the abdomen. Your doctor will discuss these risks with you before the procedure.
Do I need to prepare for this test?
Your doctor will provide specific instructions. You may need to fast for a few hours before the procedure. Inform your doctor about any medications you are taking, especially blood thinners.
What do abnormal results mean?
Abnormal basophil counts are interpreted in context with other findings from the fluid analysis. Your doctor will explain what the results mean for your specific situation and discuss any necessary follow-up tests or treatments.
  1. 1
    MedlinePlus - AscitesSource
  2. 2
    Mayo Clinic - ParacentesisSource
  3. 3
    Merck Manual - Analysis of Peritoneal FluidSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy