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Basophilsprtmanual

Published: August 9, 2026Updated: August 16, 2026

This test counts basophils (a type of white blood cell) in fluid collected from the abdominal cavity to help diagnose the cause of fluid buildup.

Quick summary

{"Basophils are white blood cells that help fight infections and allergic reactions","This test examines fluid from the abdominal cavity (ascites)","Elevated basophils may indicate infection, inflammation, or certain cancers","The test is performed manually under a microscope","Results help doctors determine the cause of abdominal fluid accumulation"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
68392-0
Short code
PERB
Synonyms
Basophils # Prt Manual
Category
chemistry
Sample
serum
Container
Sterile, leak-proof container without anticoagulant preferred; EDTA tube acceptable for cell counts. Transport in sterile conditions at room temperature or refrigerated.
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–120 years
Method
manual-count
CPT
89051
SNOMED
38678009
Specimen volume
Minimum 5 mL of peritoneal fluid; larger volumes (10-50 mL) preferred for adequate cellular analysis.
Stability
Refrigerate at 2-8°C; analyze within 1 hour of collection for optimal cell morphology. For delayed analysis, prepare smears or use preservative.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
1–4 hours
Order
routine

What is this test?

The basophil count in peritoneal fluid is a laboratory test that counts basophils in fluid collected from the abdominal cavity. Peritoneal fluid, also called ascitic fluid, is a small amount of fluid that naturally lubricates the abdominal organs. When excess fluid accumulates in the abdomen (a condition called ascites), doctors may need to analyze it to find the cause.

What are basophils?

Basophils are a type of white blood cell (leukocyte) that play important roles in the body's immune system. They are part of the body's defense against parasites, help in allergic reactions, and release substances like histamine during immune responses. While they normally make up a small percentage of white blood cells in the blood, their presence and number in peritoneal fluid can provide valuable diagnostic information.

How is the test performed?

The test requires a sample of peritoneal fluid, which is collected through a procedure called paracentesis (also known as abdominal tap). During this procedure, a doctor inserts a thin needle into the abdominal cavity to withdraw fluid. The sample is then taken to a laboratory where a technician examines it under a microscope to count the number of basophils and other cells present.

Why might I need this test?

Your doctor may order this test if you have accumulated fluid in your abdominal cavity and they need to determine the cause. Some common reasons include: liver disease (cirrhosis), heart failure, kidney disease, infections, pancreatitis, or certain types of cancer. The basophil count, along with other tests on the fluid, helps distinguish between different causes of fluid accumulation.

Reference range

Basophils are normally absent or extremely rare in peritoneal fluid; presence is reported qualitatively (rare, occasional, many) or as an absolute count.N/A

Specimen: serum

No fasting required. Patient should be prepared for paracentesis procedure per institutional protocol. Informed consent should be obtained prior to peritoneal fluid collection.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the test painful?
During the paracentesis procedure, you may feel a brief sting from the numbing injection. You may feel pressure or mild discomfort as the needle is inserted to withdraw fluid. The procedure is generally well-tolerated, and your doctor will use local anesthesia to minimize discomfort.
How long does it take to get results?
Results are typically available within 1-2 days, depending on the laboratory. The manual cell count requires careful examination under a microscope by a trained technician.
Are there any risks from the test?
Minor risks include slight bleeding, bruising, or infection at the puncture site. In rare cases, there may be leakage of fluid or injury to nearby organs. Your doctor will discuss these risks with you before the procedure.
Do I need to fast before this test?
Fasting is not typically required for this test. However, follow any specific instructions provided by your doctor, as they may have reasons to ask you to fast based on your individual situation.
What do abnormal results mean?
Abnormal basophil counts should be interpreted in the context of your overall health and other test results. Your doctor will explain what your specific results mean and whether additional testing or treatment is needed.
  1. 1
    MedlinePlus - AscitesSource
  2. 2
    Mayo Clinic - AscitesSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy