Synthetic Cannabinoids Test (Synthetic Marijuana, K2, or Spice Screening)
This test detects the presence of BB-22 (3-carboxyindole), a metabolite of synthetic cannabinoids, in your urine sample.
{"Detects use of synthetic cannabinoids (man-made cannabis-like substances)","Uses a urine sample collected at a testing facility","Positive results indicate recent synthetic cannabinoid use","Synthetic cannabinoids can be more dangerous than natural cannabis"}
Key facts at a glance
- LOINC
- 87484-2
- Short code
- BB22COOH
- Synonyms
- BB-22 3-carboxyindole Ur Ql Cfm
- Category
- chemistry
- Sample
- urine
- Container
- Sterile, tamper-evident urine collection cup; transfer to screw-cap plastic transport tube if needed
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- confirm
- CPT
- 80371
- SNOMED
- 307440008
- Specimen volume
- 30-50 mL of random urine
- Stability
- Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days; Room temperature: 3 days
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 48–120 hours
- Order
- routine
What is a synthetic cannabinoids test and why might my doctor order it for me?
The BB-22 3-carboxyindole urine test is a confirmatory laboratory test that detects the presence of a specific metabolite (3-carboxyindole) in urine. This metabolite is produced when the body processes BB-22, a synthetic cannabinoid also known as QUCHIC. Synthetic cannabinoids are man-made chemicals designed to produce effects similar to tetrahydrocannabinol (THC), the active compound in marijuana.
What are synthetic cannabinoids and how are they different from marijuana?
Synthetic cannabinoids are a class of designer drugs that mimic the effects of natural cannabis. They are often sprayed onto herbal mixtures and sold under names like "Spice," "K2," or "fake weed." These substances are not regulated and can be significantly more potent and dangerous than natural marijuana. BB-22 is one of many synthetic cannabinoids that have been developed and can cause serious health effects including severe anxiety, paranoia, hallucinations, rapid heart rate, and in some cases, kidney damage or death.
How is a synthetic cannabinoids test performed and how should I prepare for it?
A healthcare provider will collect a urine sample in a sterile container. The sample is then sent to a laboratory where it undergoes confirmatory testing using specialized methods (typically mass spectrometry) to accurately identify the presence of the 3-carboxyindole metabolite. This confirmatory method is highly specific and can distinguish synthetic cannabinoid metabolites from other substances.
What do my synthetic cannabinoids test results mean for my health and care?
A 'Positive' result indicates that BB-22 or related synthetic cannabinoids have been used recently (typically within several days to a week, depending on frequency of use). A 'Negative' result means no synthetic cannabinoid metabolite was detected at the time of testing. It is important to note that false positives are rare with confirmatory testing, but the test does not indicate the amount used or level of impairment.
Reference range
Negative (cutoff: 10 ng/mL)N/A
No fasting required. Patient should be informed that adulteration or substitution of urine specimen may affect results. A chain of custody collection may be required for forensic or workplace testing.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings2
Frequently asked questions
How long does BB-22 stay in my urine?
Can a positive result affect my employment?
Are there any medical uses for this test?
What should I do if I test positive?
Can secondhand exposure cause a positive result?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy