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Synthetic Cannabinoids Test (Synthetic Marijuana, K2, or Spice Screening)

Published: August 9, 2026Updated: August 16, 2026

This test detects the presence of BB-22 (3-carboxyindole), a metabolite of synthetic cannabinoids, in your urine sample.

Quick summary

{"Detects use of synthetic cannabinoids (man-made cannabis-like substances)","Uses a urine sample collected at a testing facility","Positive results indicate recent synthetic cannabinoid use","Synthetic cannabinoids can be more dangerous than natural cannabis"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
87484-2
Short code
BB22COOH
Synonyms
BB-22 3-carboxyindole Ur Ql Cfm
Category
chemistry
Sample
urine
Container
Sterile, tamper-evident urine collection cup; transfer to screw-cap plastic transport tube if needed
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
confirm
CPT
80371
SNOMED
307440008
Specimen volume
30-50 mL of random urine
Stability
Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days; Room temperature: 3 days
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
48–120 hours
Order
routine

What is a synthetic cannabinoids test and why might my doctor order it for me?

The BB-22 3-carboxyindole urine test is a confirmatory laboratory test that detects the presence of a specific metabolite (3-carboxyindole) in urine. This metabolite is produced when the body processes BB-22, a synthetic cannabinoid also known as QUCHIC. Synthetic cannabinoids are man-made chemicals designed to produce effects similar to tetrahydrocannabinol (THC), the active compound in marijuana.

What are synthetic cannabinoids and how are they different from marijuana?

Synthetic cannabinoids are a class of designer drugs that mimic the effects of natural cannabis. They are often sprayed onto herbal mixtures and sold under names like "Spice," "K2," or "fake weed." These substances are not regulated and can be significantly more potent and dangerous than natural marijuana. BB-22 is one of many synthetic cannabinoids that have been developed and can cause serious health effects including severe anxiety, paranoia, hallucinations, rapid heart rate, and in some cases, kidney damage or death.

How is a synthetic cannabinoids test performed and how should I prepare for it?

A healthcare provider will collect a urine sample in a sterile container. The sample is then sent to a laboratory where it undergoes confirmatory testing using specialized methods (typically mass spectrometry) to accurately identify the presence of the 3-carboxyindole metabolite. This confirmatory method is highly specific and can distinguish synthetic cannabinoid metabolites from other substances.

What do my synthetic cannabinoids test results mean for my health and care?

A 'Positive' result indicates that BB-22 or related synthetic cannabinoids have been used recently (typically within several days to a week, depending on frequency of use). A 'Negative' result means no synthetic cannabinoid metabolite was detected at the time of testing. It is important to note that false positives are rare with confirmatory testing, but the test does not indicate the amount used or level of impairment.

Reference range

Negative (cutoff: 10 ng/mL)N/A

Specimen: urine

No fasting required. Patient should be informed that adulteration or substitution of urine specimen may affect results. A chain of custody collection may be required for forensic or workplace testing.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings2

Frequently asked questions

How long does BB-22 stay in my urine?
Synthetic cannabinoids and their metabolites can typically be detected in urine for 2-7 days after use, though this varies based on factors like frequency of use, metabolism, body fat percentage, and hydration. Chronic users may test positive for longer periods.
Can a positive result affect my employment?
Many employers maintain drug-free workplace policies and may terminate employment or require completion of a substance abuse program following a positive synthetic cannabinoid test. Employment consequences depend on your employer's specific drug testing policy and the nature of your position.
Are there any medical uses for this test?
This is primarily a forensic/toxicology test used for detection purposes rather than diagnosis of medical conditions. Healthcare providers may use it to confirm synthetic cannabinoid exposure in patients presenting with unexplained symptoms consistent with synthetic cannabinoid toxicity.
What should I do if I test positive?
If you test positive and have been using synthetic cannabinoids, consider seeking help from a healthcare provider or substance abuse treatment program. Synthetic cannabinoids carry significant health risks, and professional support can help you stop use safely. If you believe the result is incorrect, discuss the possibility of retesting with your healthcare provider or the testing facility.
Can secondhand exposure cause a positive result?
It is extremely unlikely for passive secondhand exposure to synthetic cannabinoids to cause a positive result on this confirmatory test. The detection threshold is set to require actual use. However, if you believe you may have been exposed in an enclosed space to significant amounts of synthetic cannabinoid smoke, discuss this with the testing authority.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy